کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10815949 | 1058530 | 2015 | 37 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Acute Wnt pathway activation positively regulates leptin gene expression in mature adipocytes
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کلمات کلیدی
SGBSDACT1β-catSNPsLUCWnt3aTCF7L2HFDPPARγDKK1SFRP1T2DAdipocytes - آدیپوسیتinsulin - انسولینβ-catenin - بتا-کاتنینthymidine kinase - تیمیدین کینازType 2 diabetes - دیابت نوع 2high-fat diet - رژیم غذایی با چربی بالاSimpson–Golabi–Behmel syndrome - سندرم سیمپسون-گلابی-بهملtranscription factor 7-like 2 - فاکتور رونویسی 7 مانند 2luciferase - لوسیفرازLeptin - لپتین Genome-wide association studies - مطالعات مرتبط با ژنومGWAS - مطالعهٔ همخوانی سراسر ژنومSecreted frizzled-related protein 1 - پروتئین وابسته به فروتولید جدا شده 1Single nucleotide polymorphisms - پلیمورفیسم تک نوکلئوتیدیPeroxisome proliferator-activated receptor gamma - گاما گیرنده گیرنده فعال پرولیفیزوم فعال
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Genome-wide association studies (GWAS) have revealed the implication of several Wnt signaling pathway components, including its effector transcription factor 7-like 2 (TCF7L2) in diabetes and other metabolic disorders. As TCF7L2 is expressed in adipocytes, we investigated its expression and function in rodent fat tissue and mature adipocytes. We found that TCF7L2 mRNA expression in C57BL/6 mouse epididymal fat tissue was up-regulated by feeding but down-regulated by intraperitoneal insulin injection. In high-fat diet (HFD) fed mice, db/db mice and Zucker (fa/fa) rats, epididymal fat TCF7L2 mRNA levels were lower than the corresponding controls. Treating rat adipocytes with 100 nM insulin repressed TCF7L2 mRNA and protein levels, associated with the repression of leptin mRNA level. The treatment with 1 nM insulin, however, stimulated TCF7L2 and leptin mRNA levels. This stimulation could be attenuated by iCRT14, an inhibitor of β-catenin/TCF-responsive transcription. Wnt3a stimulated leptin mRNA level, which was also blocked by iCRT14 co-treatment. Utilizing the leptin-expressing cell line HTR8 as a tool, we defined an evolutionarily conserved CREB binding motif that mediated Wnt3a activation. Although Wnt activation is known to repress the differentiation of 3T3-L1 cells towards mature adipocytes, short-term Wnt3a treatment of differentiated 3T3-L1 cells stimulated leptin mRNA levels. Thus, wnt pathway plays a dual function in adipocytes, including the well-known repressive effect on adipogenesis and the stimulation of leptin production in mature adipocytes in response to nutritional status.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 27, Issue 3, March 2015, Pages 587-597
Journal: Cellular Signalling - Volume 27, Issue 3, March 2015, Pages 587-597
نویسندگان
Zong-Lan Chen, Wei-Juan Shao, Fen Xu, Ling Liu, Bei-Si Lin, Xiao-Hong Wei, Zhuo-Lun Song, Huo-Gen Lu, I. George Fantus, Jian-Ping Weng, Tian-Ru Jin,