کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10816165 | 1058543 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
5-Lipoxygenase contributes to PPARγ activation in macrophages in response to apoptotic cells
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کلمات کلیدی
5-LOLUCNACEfferocytosis5-Lipoxygenase - 5-لیپوکسیژنازMϕ - MφN-acetyl-cysteine - N-استیل سیستئینROS - ROSPPRE - ارسالFlap - برآافزا Overexpression - بیش از حد بیانSepsis - سپتیسمی یا مسمومیت خونPPAR response element - عنصر پاسخ PPARdominant negative - غالب منفی استAlternative activation - فعال سازی جایگزینluciferase - لوسیفرازmacrophage(s) - ماکروفاژ (ها)Macrophages - ماکروفاژها،درشت خوارهاMarker - نشانگرNitric oxide - نیتریک اکسیدFirefly - کرم شب تابPositive control - کنترل مثبتReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Macrophage polarization to an anti-inflammatory phenotype upon contact with apoptotic cells is a contributing hallmark to immune suppression during the late phase of sepsis. Although the peroxisome proliferator-activated receptor γ (PPARγ) supports this macrophage phenotype switch, it remains elusive how apoptotic cells activate PPARγ. Assuming that a molecule causing PPARγ activation in macrophages originates in the cell membrane of apoptotic cells we analyzed lipid rafts from apoptotic, necrotic, and living human Jurkat T cells which showed the presence of 5-lipoxygenase (5-LO) in lipid rafts of apoptotic cells only. Incubating macrophages with lipid rafts of apoptotic, but not necrotic or living cells, induced PPAR responsive element (PPRE)-driven mRuby reporter gene expression in RAW 264.7 macrophages stably transduced with a 4xPPRE containing vector. Experiments with lipid rafts of apoptotic murine EL4 T cells revealed similar results. To verify the involvement of 5-LO in activating PPARγ in macrophages, Jurkat T cells were incubated with the 5-LO inhibitor MK-866 prior to induction of apoptosis, which failed to induce mRuby expression. Similar results were obtained with lipid rafts of apoptotic EL4 T cells preexposed to the 5-LO inhibitors zileuton and CJ-13610. Interestingly, Jurkat T cells overexpressing 5-LO failed to activate PPARγ in macrophages, while their 5-LO overexpressing apoptotic counterparts did. Our results suggest that during apoptosis 5-LO gets associated with lipid rafts and synthesizes ligands that in turn stimulate PPARγ in macrophages.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 25, Issue 12, December 2013, Pages 2762-2768
Journal: Cellular Signalling - Volume 25, Issue 12, December 2013, Pages 2762-2768
نویسندگان
Andreas von Knethen, Lisa K. Sha, Laura Kuchler, Annika K. Heeg, Dominik Fuhrmann, Heinrich Heide, Ilka Wittig, Thorsten J. Maier, Dieter Steinhilber, Bernhard Brüne,