کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10847847 | 1070484 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of a novel conjugate in human urine: bile acid acyl galactosides
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
DCAHDCACDCAUGTUDCAAPCIPMP3-Methyl-1-phenyl-5-pyrazoloneESIMS/MS - MS / MSLCA - ارزیابی چرخه حیاتChenodeoxycholic acid - اسید ChenodeoxycholicDeoxycholic acid - اسید DeoxycholicBile acid - اسید صفراویLithocholic acid - اسید لیتاکولیکHyodeoxycholic acid - اسید هیودوکسوخیکلCholic acid - اسید چلیکUrsodeoxycholic acid - اورسودوکسی کولیک اسید، اورسودیولMass spectrometry - طیف سنجی جرمیTandem mass spectrometry - طیف سنجی جرمی پشت سر هم یا متوالیliquid chromatography - کروماتوگرافی مایعLiquid chromatography/mass spectrometry - کروماتوگرافی مایع / طیف سنج جرمیGalactose - گالاکتوزمی Glycoside - گلیکوزیدelectrospray ionisation - یونیزاسیون الکترو اسپریelectrospray ionization - یونیزاسیون الکترو اسپریatmospheric pressure chemical ionization - یونیزاسیون شیمیایی جوی شیمیاییAtmospheric pressure chemical ionisation - یونیزاسیون شیمیایی فشار جو
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Identification of a novel conjugate in human urine: bile acid acyl galactosides Identification of a novel conjugate in human urine: bile acid acyl galactosides](/preview/png/10847847.png)
چکیده انگلیسی
We report a novel conjugate, bile acid acyl galactosides, which exist in the urine of healthy volunteers. To identify the two unknown peaks obtained in urine specimens from healthy subjects, the specimens were subjected to solid phase extraction and then to liquid chromatographic separation. The eluate corresponding to the unknown peaks on the chromatogram was collected. Following alkaline hydrolysis and liquid chromatography (LC)/electrospray ionization (ESI)-mass spectrometric (MS) analysis, cholic acid (CA) and deoxycholic acid (DCA) were identified as liberated bile acids. When a portion of the alkaline hydrolyzate was subjected to a derivatization reaction with 1-phenyl-3-methyl-5-pyrazolone, a derivative of galactose was detected by LC/ESI-MS. Finally, the liquid chromatographic and mass spectrometric properties of these unknown compounds in urine specimens were compared to those of authentic specimens and the structures were confirmed as CA 24-galactoside and DCA 24-galactoside. These results strongly imply that bile acid 24-galactosides, a novel conjugate, were synthesized in the human body.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Steroids - Volume 70, Issue 3, March 2005, Pages 185-192
Journal: Steroids - Volume 70, Issue 3, March 2005, Pages 185-192
نویسندگان
Takaaki Goto, Akihiro Shibata, Daisuke Sasaki, Naoto Suzuki, Takanori Hishinuma, Genta Kakiyama, Takashi Iida, Nariyasu Mano, Junichi Goto,