کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10876910 | 1075787 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Reduced mitochondrial and ascorbate-glutathione activity after artificial ageing in soybean seed
ترجمه فارسی عنوان
فعالیت های میتوکندری و آسکوربات-گلوتاتیون پس از پیری مصنوعی در دانه سویا کاهش می یابد
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کلمات کلیدی
MDAmonodehydroascorbate reductaseRCRCOXGSSGMDHARdehydroascorbate reductaseDHARAPXGSHTCAASCTESBSA - BSAROS - ROSAdenosine Triphosphate - آدنوزین تری فسفاتATP - آدنوزین تری فسفات یا ATPreduced ascorbate - آسکوربات کاهش یافته استAscorbic acid - آسکوربیک اسیدbovine serum albumin - آلبومین سرم گاوtricarboxylic acid - اسید تری کربوکسیلیکTem - این استAsh - خاکسترSoybean seed - دانه سویاDHA - دوکوساهگزائنوئیک اسیدSOD - سدSuperoxide dismutase - سوکسوکس دیسموتازcytochrome c oxidase - سیتوکروم سی اکسیدازmalondialdehyde - مالون دی آلدهیدMitochondria - میتوکندریاTransmission electron microscopy - میکروسکوپ الکترونی عبوریascorbate peroxidase - پراکسیداز آسکورباتیArtificial ageing - پیری مصنوعیAscorbate–glutathione cycle - چرخه اسکوربات-گلوتاتیونGlutathione - گلوتاتیونglutathione disulfide - گلوتاتیون دی سولفیدglutathione reductase - گلوتاتیون ردوکتازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
چکیده انگلیسی
The effect of artificial ageing on the relationship between mitochondrial activities and the antioxidant system was studied in soybean seeds (Glycine max L. cv. Zhongdou No. 27). Ageing seeds for 18 d and 41 d at 40 °C reduced germination from 99% to 52% and 0%, respectively. In comparison to the control, malondialdehyde content and leachate conductivity in aged seeds increased and were associated with membrane damage. Transmission electron microscopy and Percoll density gradient centrifugation showed that aged seeds mainly contained poorly developed mitochondria in which respiration and marker enzymes activities were significantly reduced. Heavy mitochondria isolated from the interface of the 21% and 40% Percoll were analyzed. Mitochondrial antioxidant enzymes activities including superoxide dismutase, ascorbate peroxidase, glutathione reductase, monodehydroascorbate reductase, and dehydroascorbate reductase were significantly reduced in aged seeds. A decrease in total ascorbic acid (ASC) and glutathione (GSH) content as well as the reduced/oxidized ratio of ASC and GSH in mitochondria with prolonged ageing showed that artificial ageing reduced ASC-GSH cycle activity. These results suggested an elevated reactive oxygen species (ROS) level in the aged seeds, which was confirmed by measurements of superoxide radical and hydrogen peroxide levels. We conclude that mitochondrial dysfunction in artificially aged seeds is due to retarded mitochondrial and ASC-GSH cycle activity and elevated ROS accumulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Plant Physiology - Volume 171, Issue 2, 15 January 2014, Pages 140-147
Journal: Journal of Plant Physiology - Volume 171, Issue 2, 15 January 2014, Pages 140-147
نویسندگان
Xia Xin, Qian Tian, Guangkun Yin, Xiaoling Chen, Jinmei Zhang, Sophia Ng, Xinxiong Lu,