کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10876983 | 1075898 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Monitoring the stability of Rubisco in micropropagated grapevine (Vitis vinifera L.) by two-dimensional electrophoresis
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کلمات کلیدی
SDSRuBPRubisco small subunitRubisco large subunitlow CO2PMSFSSULSUIEF2-DEPFDNaATCADTTRuBisCO3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate - 3 - [(3-کلامیدوپروپیل) دی متیل آمونیو] -1-پروپان سولفوناتHigh CO2 - CO2 بالاIPG - GPAimmobilized pH gradient - gradient pH immobilizedribulose-1,5-bisphosphate - ribulose-1،5-bisphosphateribulose-1,5-bisphosphate carboxylase/oxygenase - Ribulose-1،5-Bisphosphate کربوکسیلاز / OxygenaseROS - ROSEDTA - اتیلن دی آمین تترا استیک اسید ethylene diamine tetraacetic acid - اتیلن دیامین تتراستیک اسیدtrichloroacetic acid - اسید ترشکلراکتیکTwo-dimensional electrophoresis - الکتروفورز دو بعدیSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدSDS polyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمید SDSAcclimatization - انعطاف پذیریhigh light - برجستهphoton flux density - تراکم شار فوتونisoelectric focusing - تمرکز ذره ای الکتریکیdithiothreitol - دیتیوتریتولRelative humidity - رطوبت نسبیsodium dodecylsulfate - سدیم دودسیل سولفاتPhenylmethanesulfonyl fluoride - فنیل متیل سولفونیل فلورایدlow light - نور کمCHAPS - چاپسReactive oxygen species - گونههای فعال اکسیژنVitis vinifera - گیاه انگور، مو
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Plants cultured in vitro suffer from several physiological and biochemical impairments due to the artificial conditions of growth, namely the composition of the heterotrophic media. Upon transfer to ex vitro, the higher irradiances, compared to in vitro, can lead to oxidative stress symptoms, which can be counteracted by CO2 concentrations above atmospheric levels. Here we analyse the stability of Rubisco in in vitro grapevine plantlets, and after transfer to ex vitro under four acclimatization treatments: low irradiance (LL, 150 μmol mâ2 sâ1) and high irradiance (HL, 300 μmol mâ2 sâ1) in association with CO2 concentrations of 350 (LCO2) and 700 (HCO2) μL Lâ1. Proteins were separated with SDS polyacrylamide gel electrophoresis and two-dimensional electrophoresis and Rubisco degradation peptides were analysed by immunoblotting with anti-LSU antibodies. These degradation products were present in the leaves of plantlets under both in vitro and ex vitro treatments. Under LCO2 they were maintained for almost all of the 28 days of the acclimatization period, while becoming scarcely detected after 14 days under HCO2 and after 7 days when HCO2 was associated with HL. These results appear to confirm the counteraction of HCO2 concentrations over the oxidative stress eventually caused by HL. The patterns of soluble sugars in acclimatizing leaves under HLHCO2 also gave an indication of a faster acquisition of autotrophic characteristics.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Plant Physiology - Volume 162, Issue 4, 22 April 2005, Pages 365-374
Journal: Journal of Plant Physiology - Volume 162, Issue 4, 22 April 2005, Pages 365-374
نویسندگان
LuÃsa C. Carvalho, Maria G. EsquÃvel, Isabel Martins, C. Pinto Ricardo, Sara Amâncio,