کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10903807 | 1086527 | 2015 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Assessing the osteoblast transcriptome in a model of enhanced bone formation due to constitutive Gs-G protein signaling in osteoblasts
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کلمات کلیدی
CRESynchrotron radiation micro-computed tomographyG protein coupled receptor (GPCR)Von KossaOBSGPCRTRAPBMSCsGFPFACSqPCRPCMcycle threshold - آستانه چرخهOsteoblasts - استئوبلاست هاIntramembranous bone - استخوان داخل جمجمهtartrate-resistant acid phosphatase - اسید فسفاتاز مقاوم در برابر تارتاتFourier transform infrared - تبدیل فوریه مادون قرمزGene expression analysis - تجزیه بیان ژنfluorescent activated cell sorting - دسته بندی سلول فعال فلورسنت فعال استbone marrow stromal cells - سلول های استرومایی مغز استخوانFTIR - طیف سنج مادون قرمزMouse model - مدل موشSDM - منابع انسانیquantitative real-time polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی زمان واقعی استgreen fluorescent protein - پروتئین فلورسنت سبزG protein-coupled receptor - گیرندههای جفتشونده با پروتئین جی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
G protein-coupled receptor (GPCR) signaling in osteoblasts (OBs) is an important regulator of bone formation. We previously described a mouse model expressing Rs1, an engineered constitutively active Gs-coupled GPCR, under the control of the 2.3 kb Col I promoter. These mice showed a dramatic age-dependent increase in trabecular bone of femurs. Here, we further evaluated the effects of enhanced Gs signaling in OBs on intramembranous bone formation by examining calvariae of 1- and 9-week-old Col1(2.3)/Rs1 mice and characterized the in vivo gene expression specifically occurring in osteoblasts with activated Gs G protein-coupled receptor signaling, at the cellular level rather than in a whole bone. Rs1 calvariae displayed a dramatic increase in bone volume with partial loss of cortical structure. By immunohistochemistry, Osterix was detected in cells throughout the inter-trabecular space while Osteocalcin was expressed predominantly in cells along bone surfaces, suggesting the role of paracrine mediators secreted from OBs driven by 2.3 kb Col I promoter could influence early OB commitment, differentiation, and/or proliferation. Gene expression analysis of calvarial OBs revealed that genes affected by Rs1 signaling include those encoding proteins important for cell differentiation, cytokines and growth factors, angiogenesis, coagulation, and energy metabolism. The set of Gs-GPCRs and other GPCRs that may contribute to the observed skeletal phenotype and candidate paracrine mediators of the effect of Gs signaling in OBs were also determined. Our results identify novel detailed in vivo cellular changes of the anabolic response of the skeleton to Gs signaling in mature OBs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 333, Issue 2, 1 May 2015, Pages 289-302
Journal: Experimental Cell Research - Volume 333, Issue 2, 1 May 2015, Pages 289-302
نویسندگان
Lalita Wattanachanya, Liping Wang, Susan M. Millard, Wei-Dar Lu, Dylan O'Carroll, Edward C. Hsiao, Bruce R. Conklin, Robert A. Nissenson,