کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10904533 | 1086607 | 2011 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mechanotransduction activates α5β1 integrin and PI3K/Akt signaling pathways in mandibular osteoblasts
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کلمات کلیدی
RANKLFGF23WntONJα5β1 IntegrinALP - آلکالن فسفاتازAlkaline phosphatase - آلکالین فسفاتاز یا فسفاتاز قلیاییOsteonecrosis of the jaw - استئوژنز فکMechanical loading - بارگیری مکانیکیRemodeling - بازسازیApoptosis - خزان یاختهایLow Intensity Pulsed Ultrasound - سونوگرافی پالس شده با شدت کمfibroblast growth factor 23 - فاکتور رشد فیبروبلاست 23mandible - فَکّ پایین، زیرواره یا مندیبولLIPUS - لیپوس
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Mechanotransduction activates α5β1 integrin and PI3K/Akt signaling pathways in mandibular osteoblasts Mechanotransduction activates α5β1 integrin and PI3K/Akt signaling pathways in mandibular osteoblasts](/preview/png/10904533.png)
چکیده انگلیسی
It is unclear how bone cells at different sites detect mechanical loading and how site-specific mechanotransduction affects bone homeostasis. To differentiate the anabolic mechanical responses of mandibular cells from those of calvarial and long bone cells, we isolated osteoblasts from C57B6J mouse bones, cultured them for 1 week, and subjected them to therapeutic low intensity pulsed ultrasound (LIPUS). While the expression of the marker proteins of osteoblasts and osteocytes such as alkaline phosphatase and FGF23, as well as Wnt1 and β-catenin, was equally upregulated, the expression of mandibular osteoblast messages related to bone remodeling and apoptosis differed from that of messages of other osteoblasts, in that the messages encoding the pro-remodeling protein RANKL and the anti-apoptotic protein Bcl-2 were markedly upregulated from the very low baseline levels. Blockage of the PI3K and α5β1 integrin pathways showed that the mandibular osteoblast required mechanotransduction downstream of α5β1 integrin to upregulate expression of the proteins β-catenin, p-Akt, Bcl-2, and RANKL. Mandibular osteoblasts thus must be mechanically loaded to preserve their capability to promote remodeling and to insure osteoblast survival, both of which maintain intact mandibular bone tissue. In contrast, calvarial Bcl-2 is fully expressed, together with ILK and phosphorylated mTOR, in the absence of LIPUS. The antibody blocking α5β1 integrin suppressed both the baseline expression of all calvarial proteins examined and the LIPUS-induced expression of all mandibular proteins examined. These findings indicate that the cellular environment, in addition to the tridermic origin, determines site-specific bone homeostasis through the remodeling and survival of osteoblastic cells. Differentiated cells of the osteoblastic lineage at different sites transmit signals through transmembrane integrins such as α5β1 integrin in mandibular osteoblasts, whose signaling may play a major role in controlling bone homeostasis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 317, Issue 18, 1 November 2011, Pages 2642-2649
Journal: Experimental Cell Research - Volume 317, Issue 18, 1 November 2011, Pages 2642-2649
نویسندگان
H. Watabe, T. Furuhama, N. Tani-Ishii, Y. Mikuni-Takagaki,