کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10905036 | 1086718 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The novel tumor suppressor p33ING2 enhances UVB-induced apoptosis in human melanoma cells
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کلمات کلیدی
PBSp53PVDFSRBIngAntisense - آنتیسنسUVB irradiation - اشعه UVBUltraviolet - اشعه فرابنفشInhibitor of growth - بازدارنده رشدMelanoma - خال سرطانی یا ملانوماApoptosis - خزان یاختهایpolyvinylidene difluoride - دی فلوئورید پلی وینیلیدینTumor suppressor - سرکوبگر تومورsulforhodamine B - سولفوردامین بPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریPropidium iodide - پروتئین یدید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The roles of p33ING2 as a tumor suppressor candidate have been shown through regulation of gene transcription, induction of cell cycle arrest, and apoptosis. As p33ING2 shares 58.9% homology with p33ING1b, we hypothesized that p33ING2 shares functional similarities with p33ING1b. We previously found that p33ING1b cooperates with p53 to enhance UVB-induced apoptosis. Here, we report that overexpression of p33ING2 enhanced apoptosis in UVB-irradiated and non-irradiated melanoma MMRU cells. We demonstrate that enhancement of apoptosis by p33ING2 requires the presence of functional p53. Furthermore, we found that overexpression of p33ING2 significantly downregulated the expression of Bcl-2 after UVB irradiation, resulting in an increased Bax/Bcl-2 ratio. Moreover, we found that p33ING2 promoted Bax translocation to mitochondria, altered the mitochondrial membrane potential, and induced cytochrome c release and thus the activation of caspases 9 and 3. In addition, we showed that under non-stress conditions p33ING2 upregulates Fas expression and activates caspase 8. Taken together, we demonstrate that p33ING2 cooperates with p53 to regulate apoptosis via activation of both the mitochondrial/intrinsic and death-receptor/extrinsic apoptotic pathways.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 304, Issue 2, 1 April 2005, Pages 531-543
Journal: Experimental Cell Research - Volume 304, Issue 2, 1 April 2005, Pages 531-543
نویسندگان
Mei Yieng Chin, Kin Cheung P. Ng, Gang Li,