کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10905194 | 1086737 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Processing of the human protocadherin Fat1 and translocation of its cytoplasmic domain to the nucleus
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کلمات کلیدی
PBSPMSFNLSA.U.phorbol 12-myristate 13-acetateCFGPMA - LDC هاNuclear translocation - انتقال هسته ایnuclear localization sequence - دنباله محلی سازی هسته ایDevelopment - رشدphenylmethylsulphonyl fluoride - فنیل متیل سولفونیل فلورایدphosphate-buffered saline solution - محلول نمک فسفات بافرarbitrary units - واحد دلخواهRIP - پاره کردنIntramembrane proteolysis - پروتئولیت اینترمیبریprotocadherin - پروتکلادیرین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Processing of the human protocadherin Fat1 and translocation of its cytoplasmic domain to the nucleus Processing of the human protocadherin Fat1 and translocation of its cytoplasmic domain to the nucleus](/preview/png/10905194.png)
چکیده انگلیسی
The giant protein hFat1, a member of the cadherin superfamily, has been proposed to play roles in cerebral development, glomerular slit formation, and also to act as a tumor suppressor, but its mechanisms of action have not been elucidated. To examine functions of the transmembrane and cytoplasmic domains, they were expressed in HEK293 and HeLa cells as chimeric proteins in fusion with EGFP and extracellular domains derived from E-cadherin. Proteins comprising the transmembrane domain localized to the membrane fraction. Deletion of this domain resulted in a predominantly nuclear localization of the cytoplasmic segment of hFat1. Nuclear localization was largely reduced by deletion of a presumed juxta-membrane NLS. Fusion proteins located in the plasma membrane underwent proteolytic processing. In a first proteolytic step, only the extracellular domain was cleaved off. In another step, the cleavage product was released to the cytosol and was also found in a low speed pellet fraction, in accordance with the nuclear localization of the cytoplasmic domain of hFat1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 307, Issue 1, 1 July 2005, Pages 100-108
Journal: Experimental Cell Research - Volume 307, Issue 1, 1 July 2005, Pages 100-108
نویسندگان
Thomas Magg, Dietmar Schreiner, Gonzalo P. Solis, Ernesto G. Bade, Hans Werner Hofer,