کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10905203 | 1086737 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evidence that membrane transduction of oligoarginine does not require vesicle formation
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PBSNH4ClOligoarginineE-64RFUMTPα-MEM4-(2-aminoethyl)-benzenesulfonyl fluoride hydrochlorideAEBSFPMSFHEPESFITCCPP4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid - 4- (2-hydroxyethyl) -1-piperazineethanesulfonic acidfluorescein 5′-isothiocyanate - fluorescein 5'-isothiocyanateα-minimum essential medium - α حداقل محیط ضروریEndocytosis - آندوسیتوز یا درون بریEDTA - اتیلن دی آمین تترا استیک اسید Membrane transduction - انتقال غشاءQuantitative measurement - اندازه گیری کمیCho - برایChinese Hamster Ovary - تخمدان هامستر چینیphenyl-methylsulfonyl fluoride - فنیل متیل سولفونیل فلورایدPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریrelative fluorescent units - واحدهای فلورسنت نسبیHIV-1 - ویروس اچ آی وی نوع یکHuman immunodeficiency virus type 1 - ویروس نقص ایمنی بدن نوع 1Cell-penetrating peptide - پپتید نفوذ سلولیcell-penetrating peptides - پپتیدهای نفوذ سلولیAmmonium chloride - کلرید آمونیوم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Evidence that membrane transduction of oligoarginine does not require vesicle formation Evidence that membrane transduction of oligoarginine does not require vesicle formation](/preview/png/10905203.png)
چکیده انگلیسی
The involvement of vesicular formation processes in the membrane transduction and nuclear transport of oligoarginine is currently a subject of controversy. In this report, a novel quantitative method which allows for the selective measurement of membrane transduction excluding concurrent endocytosis was used to determine the effects of temperature, endosomal acidification, endosomolysis, and several known inhibitors of endocytic pathways on the internalization of oligoarginine. The results show that, unlike endocytosis, transduction of oligoarginine was not affected by incubation at 16°C as compared to the 37°C control, and was only partially inhibited at 4°C incubation. Additionally, membrane transduction was not inhibited to the same extent as endocytosis following treatment with ammonium chloride, hypertonic medium, amiloride, or filipin. The endosomolytic activity of oligoarginine was investigated by examining the leakage of FITC-dextran into the cytosolic compartment, which was not higher in the presence of oligoarginine. Furthermore, ammonium chloride showed no effect on the nuclear transport of oligoarginine. The data presented in this report indicate that membrane transduction is likely to occur at the plasma membrane without the formation of membrane vesicles, and the nuclear localization involves membrane transduction, rather than endocytosis of oligoarginine.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 307, Issue 1, 1 July 2005, Pages 164-173
Journal: Experimental Cell Research - Volume 307, Issue 1, 1 July 2005, Pages 164-173
نویسندگان
Jennica L. Zaro, Wei-Chiang Shen,