کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10905754 | 1086767 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Proliferative effects of apical, but not basal, matrix metalloproteinase-7 activity in polarized MDCK cells
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کلمات کلیدی
Polarized epithelial cellsHB-EGFECMMDCKuPADTTFBSDMEMAPMAMMP-7MMP4-aminophenylmercuric acetate - 4-آمینوفنیل متیل کربنی استات5-bromo-2′-deoxyuridine - 5-bromo-2'-deoxyuridineDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoBrdU - بروموداکسی اوریدینtumor necrosis factor alpha - تومور نکروز عامل آلفاCell proliferation - تکثیر سلولیMin - حداقلdithiothreitol - دیتیوتریتولTIMP - زمانfetal bovine serum - سرم جنین گاوHeparin-binding epidermal growth factor - عامل رشد اپیدرمی اتصال هپارینTNF-α - فاکتور نکروز توموری آلفاurokinase plasminogen activator - فعال کننده پلاسمینوژن یوروکینازMatrilysin - ماتریسینExtracellular matrix - ماتریکس خارج سلولیmatrix metalloproteinase - ماتریکس متالوپروتئینازTissue inhibitor of metalloproteinase - مهار کننده های متالوپروتئیناز بافتmultiple intestinal neoplasia - نئوپلاسی چندین رودهPARs - پارس هاMadin-Darby canine kidney - کلیه های سگ کوچولو Madin-Darbyprotease-activated receptors - گیرنده فعال فعال پروتئاز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Matrix metalloproteinase-7 (MMP-7) is primarily expressed in glandular epithelium. Therefore, its mechanism of action may be influenced by its regulated vectorial release to either the apical and/or basolateral compartments, where it would act on its various substrates. To gain a better understanding of where MMP-7 is released in polarized epithelium, we have analyzed its pattern of secretion in polarized MDCK cells expressing stably transfected human MMP-7 (MDCK-MMP-7), and HCA-7 and Caco2 human colon cancer cell lines. In all cell lines, latent MMP-7 was secreted to both cellular compartments, but was 1.5- to 3-fold more abundant in the basolateral compartment as compared to the apical. However, studies in the MDCK system demonstrated that MMP-7 activity was 2-fold greater in the apical compartment of MDCK-MMP-7HIGH-polarized monolayers, which suggests the apical co-release of an MMP-7 activator. In functional assays, MMP-7 over-expression increased cell saturation density as a result of increased cell proliferation with no effect on apoptosis. Apical MMP-7 activity was shown to be responsible for the proliferative effect, which occurred, as demonstrated by media transfer experiments, through cleavage of an apical substrate and not through the generation of a soluble factor. Taken together, our findings demonstrate the importance of MMP-7 secretion in relation to its mechanism of action when expressed in a polarized epithelium.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 303, Issue 2, 15 February 2005, Pages 308-320
Journal: Experimental Cell Research - Volume 303, Issue 2, 15 February 2005, Pages 308-320
نویسندگان
Permila C. Harrell, Lisa J. McCawley, Barbara Fingleton, J. Oliver McIntyre, Lynn M. Matrisian,