کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10964296 | 1102710 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
TLR7/8 agonists activate a mild immune response in rabbits through TLR8 but not TLR7
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کلمات کلیدی
NF-κBPBSIL-6IRF7MYD88HEKSpecies-specific recognitionGAPDHLRRIFN-βTLRIFN-γFCSInnate immunity - ایمنی ذاتیInterferon-β - اینترفرون-βinterferon-γ - اینترفرون-γinterleukin 6 - اینترلوکین 6Leucine-rich repeats - تکرار غنی از لوسینTIR - تیرToll-like receptor - تیالآرfetal calf serum - سرم گوساله جنینmyeloid differentiation factor 88 - عامل تمایز میلوئید 88Interferon regulatory factor 7 - عامل تنظیمی اینترفرون 7nuclear factor-κB - فاکتور هسته ای κBphosphate buffer saline - فسفات بافر شورAdjuvant activity - فعالیت ادجوانتهhuman embryonic kidney - کلیه جنین انسانglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژنازpattern recognition receptor - گیرنده شناسایی الگو
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: TLR7/8 agonists activate a mild immune response in rabbits through TLR8 but not TLR7 TLR7/8 agonists activate a mild immune response in rabbits through TLR8 but not TLR7](/preview/png/10964296.png)
چکیده انگلیسی
Toll-like receptors 7 (TLR7) and 8 (TLR8) recognize viral single-stranded RNA and small molecular weight agonists to activate anti-viral immune responses. TLR8s from different species have distinct ligand recognitions. For example, human TLR8 is responsive to ligand stimulation, but mouse and rat TLR8 are activated by small molecular weight agonists only in the presence of polyT-oligodeoxynucleotides. TLR7 and TLR8 have been reported to be absent and pseudogenized, respectively, in rabbit (Oryctolagus cuniculus). In this study, we detected the expression of rabbit (rab)TLR8 in immune-cell-associated tissues. Cell proliferation and cytokine expressions in rabbit splenocytes were induced by the TLR7/8 ligand but not by the TLR7 ligands, suggesting that rabTLR8 is functional but rabTLR7 is not. In rabbits, CL075, a TLR7/8 ligand, activated an antigen-specific antibody response, although one not as potent as aluminum salt or Freund's adjuvant. Nevertheless, CL075, alone or in combination with aluminum salt, generates fewer adverse effects than Freund's adjuvant at the injection sites. To further investigate the activation of rabTLR8, we cloned its cDNA. In cell-based assay, this rabTLR8 is activated by TLR7/8 ligand but not activated by TLR7 ligand. Upon stimulation the rabTLR8 had a lower activation compared to the activation of TLR8 from other species, except the mouse and rat TLR8s. Using different deletion and human-rabbit chimeric TLR8 expressing constructs, we showed that an extra peptide in the undefined region results in reduced activity of rabTLR8. These results provide a molecular basis for the mild activities of TLR7/8 ligands in rabbits, and suggest TLR7/8 agonists may provide safer immune stimuli in rabbits than in other non-rodent species.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 32, Issue 43, 29 September 2014, Pages 5593-5599
Journal: Vaccine - Volume 32, Issue 43, 29 September 2014, Pages 5593-5599
نویسندگان
Chao-Yang Lai, Yi-Ling Liu, Guann-Yi Yu, Ming-Chei Maa, Tzeng-Horng Leu, Congfeng Xu, Yunping Luo, Rong Xiang, Tsung-Hsien Chuang,