کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10966112 | 1102769 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Activation of the NLRP3 inflammasome by vault nanoparticles expressing a chlamydial epitope
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کلمات کلیدی
DAMPsASCPAMPsNLRC. trachomatisphorbol 12-myristate 13-acetateMSUPMA - LDC هاinflammasomes - التهابVaults - اموالSyk - بیمارdanger associated molecular patterns - خطرات مولکولی مرتبط با خطرcaspase recruitment domain - دامنه استخدام کاسپسspleen tyrosine kinase - طرز تیروزین کینازLysosomes - لیزوزوم هاMOMP - ماموریتMonosodium urate - مونوسیدیم اوراتpathogen associated molecular patterns - پاتوژن الگوی مولکولی مرتبط استMajor outer membrane protein - پروتئین غشای خارجی بیرونیapoptosis-associated speck-like protein containing a CARD - پروتئین پراکنده مرتبط با آپوپتوز حاوی یک کارتCARD - کارتChlamydia - کلامیدیا Chlamydia trachomatis - کلامیدیا تراکوماتیسNod-like receptor - گیرنده Nod مانند
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Activation of the NLRP3 inflammasome by vault nanoparticles expressing a chlamydial epitope Activation of the NLRP3 inflammasome by vault nanoparticles expressing a chlamydial epitope](/preview/png/10966112.png)
چکیده انگلیسی
The full potential of vaccines relies on development of effective delivery systems and adjuvants and is critical for development of successful vaccine candidates. We have shown that recombinant vaults engineered to encapsulate microbial epitopes are highly stable structures and are an ideal vaccine vehicle for epitope delivery which does not require the inclusion of an adjuvant. We studied the ability of vaults which were engineered for use as a vaccine containing an immunogenic epitope of Chlamydia trachomatis, polymorphic membrane protein G (PmpG), to be internalized into human monocytes and behave as a “natural adjuvant”. We here show that incubation of monocytes with the PmpG-1-vaults activates caspase-1 and stimulates IL-1β secretion through a process requiring the NLRP3 inflammasome and that cathepsin B and Syk are involved in the inflammasome activation. We also observed that the PmpG-1-vaults are internalized through a pathway that is transiently acidic and leads to destabilization of lysosomes. In addition, immunization of mice with PmpG-1-vaults induced PmpG-1 responsive CD4+ cells upon re-stimulation with PmpG peptide in vitro, suggesting that vault vaccines can be engineered for specific adaptive immune responses. We conclude that PmpG-1-vault vaccines can stimulate NLRP3 inflammasomes and induce PmpG-specific T cell responses.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 33, Issue 2, 3 January 2015, Pages 298-306
Journal: Vaccine - Volume 33, Issue 2, 3 January 2015, Pages 298-306
نویسندگان
Ye Zhu, Janina Jiang, Najwane Said-Sadier, Gale Boxx, Cheryl Champion, Ashley Tetlow, Valerie A. Kickhoefer, Leonard H. Rome, David M. Ojcius, Kathleen A. Kelly,