In vitro evaluation of human hybrid cell lines generated by fusion of B-lymphoblastoid cells and ex vivo tumour cells as candidate vaccines for haematological malignancies

► We fused HMy2 cells with ex vivo haematological tumour cells in vitro. ► The fusions generated a panel of stable, self replicating hybrid cell lines. ► The hybrid cell lines induced tumour-specific cytotoxic T cell responses in vitro. ► HMy2/tumour hybrid cell lines represent candidate cancer vaccines.