Role of DNA topology in uptake of polyplex molecules by dendritic cells

► This novel study quantifies the effect of DNA topology on polyplex uptake in DCs. ► Polyplex uptake was monitored by microscopy and gene expression assays. ► Complexes containing supercoiled DNA associated most efficiently with DC nuclei. ► Gene expression showed quantitative dependence on DNA topology. ► DC phenotype was independent of DNA topology.