Schistosoma japonicum calcium-binding tegumental protein SjTP22.4 immunization confers praziquantel schistosomulumicide and antifecundity effect in mice

► We cloned and characterized TP22.4, a novel tegumental protein in S. japonicum. ► Circular dichroism measurement demonstrates SjTP22.4 has Ca2+ binding ability. ► TP22.4 immunization showed a decrease in liver pathology in challenge experiment. ► SjTP22.4 combined with praziquantel showed more significant protection. ► SjTP22.4 with PZQ is of value in killing schistosomulum.