کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1168170 | 960578 | 2010 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Determination of cyanide exposure by gas chromatography–mass spectrometry analysis of cyanide-exposed plasma proteins Determination of cyanide exposure by gas chromatography–mass spectrometry analysis of cyanide-exposed plasma proteins](/preview/png/1168170.png)
Exposure to cyanide can occur in a variety of ways, including exposure to smoke from cigarettes or fires, accidental exposure during industrial processes, and exposure from the use of cyanide as a poison or chemical warfare agent. Confirmation of cyanide exposure is difficult because, in vivo, cyanide quickly breaks down by a number of pathways, including the formation of both free and protein-bound thiocyanate. A simple method was developed to confirm cyanide exposure by extraction of protein-bound thiocyanate moieties from cyanide-exposed plasma proteins. Thiocyanate was successfully extracted and subsequently derivatized with pentafluorobenzyl bromide for GC–MS analysis. Thiocyanate levels as low as 2.5 ng mL−1 and cyanide exposure levels as low as 175 μg kg−1 were detected. Samples analyzed from smokers and non-smokers using this method showed significantly different levels of protein-bound thiocyanate (p < 0.01). These results demonstrate the potential of this method to positively confirm chronic cyanide exposure through the analysis of protein-bound cyanide in human plasma.
Journal: Analytica Chimica Acta - Volume 677, Issue 1, 10 September 2010, Pages 24–28