Proteomic analysis of secreted proteins from skeletal muscle cells during differentiation

• We evaluated the dynamics of myokines during skeletal muscle differentiation.
• Proliferating myoblasts released myokines that suppress neurogenesis or adipogenesis.
• Young myotubes secreted myokines promoting myogenesis, neurogenesis or angiogenesis.
• Skeletal muscle cells secrete distinct types of myokines during differentiation.

Myokines are muscle-secreted factors to regulate cellular functions. However, it remains elusive what type of myokine is released during muscle differentiation. Here we evaluated the dynamics of myokines. More than 400 proteins were detected in conditioned medium and approximately 8% of them were categorized as myokines. The levels of myokines which promote myotube formation, vascularization or neurogenesis peaked during early differentiation, whereas myokines contributing to repellent activity against nerve cells or suppression of adipogenesis decreased after differentiation. Our findings suggest that muscle cells secrete different types of myokines at different developmental stages to communicate with various types of cells.

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