کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1198993 | 1493517 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Scale-up in centrifugal partition chromatography: The “free-space between peaks” method
ترجمه فارسی عنوان
مقیاس کروماتوگرافی پارتیشن گریز از مرکز: یک فضای آزاد بین قله روش
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کلمات کلیدی
کروماتوگرافی پارتیشن گریز از مرکز، کروماتوگرافی ضد جریان، افزایش مقیاس، کروماتوگرافی آماده سازی بهره وری،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Centrifugal Partition Chromatography (CPC) is a purification technique using a biphasic liquid system. As a preparative separation technique, scale-up is of primary concern. Once the separation is optimized on a lab-scale instrument, the scale-up transfer is quite straightforward simply using the instrument volume ratio as the linear transfer factor, thanks to the absence of solid support. Such linear transfer underestimates the performances of large-scale CPC rotors that are usually better than that of small rotors. It means that more material than predicted by the linear estimation could be purified. A fully practical method based on experimental observations is proposed. The first step is to determine experimentally the free space volume available between the two peaks of interest doing two analytical separations, one with the small analytical CPC instrument, giving ÎV1, and the second with the large preparative one, giving ÎV2. The second step is to determine on the small CPC instrument how much material can be loaded to reach the maximum mass load still giving the required purity and recovery ratio of the desired compound. Then, an accurate prediction of the maximal quantity of sample that the large-scale rotor can purify is simply obtained by multiplying the maximum mass load on the analytical CPC instrument by the free-space between peaks ÎV2/ÎV1 ratio. For demonstration purposes, the method is applied to the transfer of the CPC separation of a synthetic three-GUESS-compound mixture from a 35Â mL-rotor to a semi-prep 239-mL rotor. The paper addresses also the operating condition optimization depending on industrial production strategy (maximal load per run or maximal productivity).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1409, 28 August 2015, Pages 70-78
Journal: Journal of Chromatography A - Volume 1409, 28 August 2015, Pages 70-78
نویسندگان
Elodie Bouju, Alain Berthod, Karine Faure,