کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1201442 1493648 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of preservatives with different polarities in beverage samples by dual-phase dual stir bar sorptive extraction combined with high-performance liquid chromatography
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Analysis of preservatives with different polarities in beverage samples by dual-phase dual stir bar sorptive extraction combined with high-performance liquid chromatography
چکیده انگلیسی

A new concept of “dual-phase dual stir bar sorptive extraction (SBSE)” was proposed to simultaneously extract six preservatives with different polarities (log Ko/w values of 1.27–3.41), namely, benzoic acid (BA), sorbic acid (SA), methyl p-hydroxybenzoate (MP), ethyl p-hydroxybenzoate (EP), propyl p-hydroxybenzoate (PP), and butyl p-hydroxybenzoate (BP). The dual-phase dual SBSE apparatus was consisted of two differently coated stir bars, a 3-aminopropyltriethoxysilane (APTES)-hydroxy-terminated silicone oil (OH-TSO)-coated stir bar that was prepared by sol–gel technique and a C18 silica (C18)-polydimethylsiloxane (PDMS)-coated stir bar that was prepared by adhesion. In dual-phase dual SBSE, the two stir bars with different coatings were placed in the same sample solution for the simultaneous extraction of the target analytes with different polarities, and then the bars were desorbed in the same desorption solvent. The extraction performance of the dual-phase dual SBSE for the six preservatives was evaluated by comparing with the conventional SBSE (individual stir bar) with different coatings, including commercial PDMS, homemade PDMS, C18-APTES–OH-TSO, APTES–OH-TSO, and C18-PDMS. The experimental results showed that the dual-phase dual SBSE had the highest extraction efficiency for the six target preservatives. Based on this fact, a novel method by combining the dual-phase dual SBSE which was consisted of the APTES–OH-TSO-coated and C18-PDMS-coated stir bars with high-performance liquid chromatography-ultraviolet detection (HPLC–UV) was developed for the simultaneous analysis of six target beverage preservatives in beverages. Under optimal conditions, the limits of detection (LODs) for six target preservatives ranged from 0.6 to 2.7 μg L−1 with the relative standard deviations (RSDs) of 4.6–9.2% (CBA,SA = 5 μg L−1, CMP = 20 μg L−1, CEP,PP,BP = 10 μg L−1, n = 7). The enrichment factors (EFs) were approximately 16–42-fold (theoretical EF was 50-fold). The proposed method was validated by the analysis of six target preservatives in three kinds of beverage samples, and the recoveries for the spiked samples were in the range of 76.6–118.6% for cola, 74.6–17.5% for orange juice, and 83.0–119.1% for herbal tea, respectively.


► A new concept of “dual-phase dual stir bar sorptive extraction” was proposed.
► Six preservatives with different polarities were simultaneously analyzed.
► The dual-phase dual SBSE had the high extraction efficiency.
► The proposed method was sensitive, low-cost, simple, and efficient.
► The proposed method was applied for preservatives analysis in beverage samples.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1278, 22 February 2013, Pages 8–15
نویسندگان
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