کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1202152 1493665 2012 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Investigation of protocols to extraction and quantification of folates in vegetables matrices split into liquor and fiber fraction using factorial design
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Investigation of protocols to extraction and quantification of folates in vegetables matrices split into liquor and fiber fraction using factorial design
چکیده انگلیسی

The main protocols of extraction were investigated for the six folate forms in vegetable matrices, treated in two fractions, liquor and fiber. In a pilot study, it was used ammonium acetate added of 2-mercaptoetanol and ascorbic acid as extraction solution. The condition of use of protease and folate conjugase was evaluated, besides alternative treatments without enzyme use. Based on the results of this stage, it was built the factorial design 24, with three replications at the central point, using the following variables: temperature, time for reaction, molar concentration of the extraction solution and ratio sample/solution as independent variables and dependent variable, the amount of each folate form extracted as well as spectral and chromatographic parameters. In the pilot study it was verified that the enzyme use can cause an increase in the variability of the folate content, which enabled to build the factorial design without the enzyme use. The binomial time and temperature showed greatest impact on the extraction profile, besides high concentrations of ammonium acetate resulting in bifurcation of some peaks. 5-Methyltetrahydrofolate was extracted primordially in the liquor fraction, indicating that this treatment on the matrix provoked suitable extraction condition to this folate.


► Statistic factorial design confirms the binomial impact of time and temperature.
► Low temperature and short time are indicated as the best extraction conditions.
► 5-MTHF was suitability extracted from liquor fraction of all vegetable matrices.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1260, 19 October 2012, Pages 33–41
نویسندگان
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