کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1207520 965270 2009 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of lignans in flaxseed using liquid chromatography with time-of-flight mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of lignans in flaxseed using liquid chromatography with time-of-flight mass spectrometry
چکیده انگلیسی

A new method of flaxseed-derived lignan determination was developed using HPLC with high-resolution time-of-flight MS (TOF-MS), optimized, and compared to two existing methods (HPLC/MS/MS and GC/MS). The limits of detection (LODs) for HPLC/TOF-MS (0.002–0.043 pg) were comparable with those of the optimized and improved HPLC/MS/MS (0.001–0.015 pg), whereas the LODs for the optimized GC/MS were higher (0.02–3.0 pg, yet lower than reported before). Using the newly developed detection and separation methods, several key flaxseed sample preparation parameters (including extraction, hydrolysis, and sample purification) were evaluated resulting in the development of efficient protocol for lignan quantification from flaxseed hulls and embryos. The results confirmed the importance of quantification of both aglycones and unhydrolyzed glucosides in order to obtain the total lignan estimates.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1216, Issue 2, 9 January 2009, Pages 217–229
نویسندگان
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