کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1207665 965276 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of automated sample preparation, retention time locked gas chromatography–mass spectrometry and data analysis methods for the metabolomic study of Arabidopsis species
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Evaluation of automated sample preparation, retention time locked gas chromatography–mass spectrometry and data analysis methods for the metabolomic study of Arabidopsis species
چکیده انگلیسی

In this paper, automated sample preparation, retention time locked gas chromatography–mass spectrometry (GC–MS) and data analysis methods for the metabolomics study were evaluated. A miniaturized and automated derivatisation method using sequential oximation and silylation was applied to a polar extract of 4 types (2 types × 2 ages) of Arabidopsis thaliana, a popular model organism often used in plant sciences and genetics. Automation of the derivatisation process offers excellent repeatability, and the time between sample preparation and analysis was short and constant, reducing artifact formation. Retention time locked (RTL) gas chromatography–mass spectrometry was used, resulting in reproducible retention times and GC–MS profiles. Two approaches were used for data analysis. XCMS followed by principal component analysis (approach 1) and AMDIS deconvolution combined with a commercially available program (Mass Profiler Professional) followed by principal component analysis (approach 2) were compared. Several features that were up- or down-regulated in the different types were detected.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1218, Issue 21, 27 May 2011, Pages 3247–3254
نویسندگان
, , , , , , , ,