کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1210406 | 1493773 | 2006 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation of fusion between liposomes and erythrocytes for intracellular derivatization of amino acids in cells
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Evaluation of fusion between liposomes and erythrocytes for intracellular derivatization of amino acids in cells Evaluation of fusion between liposomes and erythrocytes for intracellular derivatization of amino acids in cells](/preview/png/1210406.png)
چکیده انگلیسی
Erythrocytes were fused with liposome for intracellular derivatization of amino acids in cells. The fusion efficiency was evaluated with capillary electrophoresis (CE) and laser-induced fluorescence (LIF) detection. Reagent fluorescein isothiocyanate (FITC) was enveloped in liposomes and introduced into erythrocytes by fusion between liposomes and erythrocytes. The amino acids in the fused cells were derivated by the introduced FITC and the derivated amino acids were extracted for detection by capillary electrophoresis equipped with laser-induced fluorescence detector. The fusion conditions were investigated. It was found that incubation of liposome and erythrocytes in the presence of 13% polyethylene glycol 6000 (PEG 6000) for 15Â min produced the highest fusion efficiency and kept the erythrocytes stability.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1110, Issues 1â2, 31 March 2006, Pages 81-85
Journal: Journal of Chromatography A - Volume 1110, Issues 1â2, 31 March 2006, Pages 81-85
نویسندگان
Hua Zhang, Juan Du, Jie Zhao, Yue-zhong Li,