Dynamic affinity chromatography in the separation of sulfated lignins binding to thrombin

► Sulfated LMWL fractions were isolated by dynamic thrombin affinity chromatography. ► The fractions differ significantly in their biophysical and biochemical properties. ► One fraction of the three studied exhibited potent antagonism of clotting. ► This fraction selectively attenuated the function of extrinsic pathway. ► Identified the 1st LMWL structure that exhibits antagonism of the extrinsic pathway.