کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1215828 | 966984 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Radiochromatographic assay of metabolites of the oostatic peptide labeled in different positions of the peptide chain
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کلمات کلیدی
TFAFAB MSRadio-HPLCtBuFMOCAAAHOBttert-butylAcOHDMFACNDCMDICdimethylacetamideDMA1-hydroxybenzotriazole - 1-هیدروکسی بنزوتریازولN,N-dimethylformamide - N، N-dimethylformamideAcetonitrile - استونیتریلTrifluoroacetic acid - اسید TrifluoroaceticAcetic acid - اسید استیکAmino acid analysis - تجزیه اسید آمینهDichloromethane - دیکلورمتانRadiolabeling - رادیو اکتیوPeptide synthesis - سنتز پپتیدfast atom bombardment mass spectrometry - طیف سنجی جرم اتم بوت شدن سریع اتم
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Reversed-phase high-performance liquid radio-chromatography (radio-HPLC) was set up to detect the time course of labeled degradation product formation of the pentapeptide H-Tyr-Asp-Pro-Ala-Pro-OH (5P), which has oostatic effects in different insect species. The detection limit of the system was in the range of 80-150Â Bq. To follow formation of the degradation products, three amino acid residues in 5P were independently tritiated: Tyr1, Pro3 and Pro5. Each of the three tritiated peptides was analyzed after incubation with fresh hemolymph or ovaries of Neobellieria bullata. In the incubation mixture, free terminal amino acids and shortened sequences of 5P were identified. A metabolite of tyrosine represented the only exception; it was finally identified as water using degradation of [3H]Tyr by tyrosinase. Metabolic degradation of [3H]Tyr-5P was found to be considerably quicker than that of H-[3H]Tyr-Asp-Pro-Ala-OH (4P). The degradation of 5P was considerably slower in ovaries in comparison to hemolymph.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 848, Issue 2, 1 April 2007, Pages 258-263
Journal: Journal of Chromatography B - Volume 848, Issue 2, 1 April 2007, Pages 258-263
نویسندگان
Richard Tykva, Jan HlaváÄek, VÄra Vlasáková, Bohuslav Äerný, Lenka BoroviÄková, Blanka Bennettová, Josef HolÃk, JiÅina Slaninová,