کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1223508 | 967893 | 2008 | 9 صفحه PDF | دانلود رایگان |
Out of three methods for the analysis of josamycin, the best one was selected and used as starting point for further development. A central composite design was applied to find the most influencing parameters and to optimize the chromatographic conditions and a full factorial design was used to perform a robustness study. The final method uses a Hypersil ODS column 5 μm, 250 mm × 4.6 mm i.d. maintained at 45 °C. The mobile phase is composed of acetonitrile–phosphate buffer (pH 3, 0.2 mol l−1)–tetrabutylammonium hydrogen sulphate 0.2 mol l−1–water (21:5:3:71, v/v/v/v). Strongly retained impurities after the main peak require gradient elution, which is obtained by increasing linearly the acetonitrile concentration (from 21% to 50%, v/v) and decreasing the TBA concentration (from 3% to 0%, v/v) in the mobile phase. The total run time was 65 min. UV detection is performed at 232 nm and the flow rate is 1 ml/min. The method shows good selectivity, precision, linearity and sensitivity. Five commercial bulk samples were analyzed.
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 48, Issue 2, 29 September 2008, Pages 347–355