کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1248456 | 1495911 | 2014 | 11 صفحه PDF | دانلود رایگان |
• Two-dimensional gel electrophoresis (2-DE) is widely used in proteomics.
• 2-DE is gaining in popularity.
• Numerous analytical and bioinformatics techniques may be coupled to 2-DE.
• New standards in proteomics are established by 2-DE.
Two-dimensional gel electrophoresis (2-DE) has become the most widely used separation tool in proteomic analysis. 2-DE is especially useful in expression proteomics, where comparative analysis of the expression of proteins exposed to environmental factors and those physiologically undisturbed is the essence of research. A comparative analysis of changes in protein expression provides information that may be utilized (e.g., in drug design and biomarker research). Furthermore, the technique of 2-DE is gaining popularity due to the possibility of coupling it with numerous analytical techniques and bioinformatics methods.
Journal: TrAC Trends in Analytical Chemistry - Volume 53, January 2014, Pages 167–177