کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1407420 1501906 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Interaction of zanamivir with DNA and RNA: Models for drug–DNA and drug–RNA bindings
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Interaction of zanamivir with DNA and RNA: Models for drug–DNA and drug–RNA bindings
چکیده انگلیسی

Zanamivir (ZAN) is the first of a new generation of influenza virus-specific drugs known as neuraminidase inhibitors, which acts by interfering with life cycles of influenza viruses A and B. It prevents the virus spreading infection to other cells by blocking the neuraminidase enzyme present on the surface of the virus. The aim of this study was to examine the stability and structural features of calf thymus DNA and yeast RNA complexes with zanamivir in aqueous solution, using constant DNA or RNA concentration (12.5 mM) and various zanamivir/polynucleotide (P) ratios of 1/20, 1/10, 1/4, and 1/2. FTIR and UV–visible spectroscopy are used to determine the drug external binding modes, the binding constant and the stability of zanamivir–DNA and RNA complexes in aqueous solution. Structural analysis showed major interaction of zanamivir with G-C (major groove) and A-T (minor groove) base pairs and minor perturbations of the backbone PO2 group with overall binding constants of Kzanamivir–DNA = 1.30 × 104 M−1 and Kzanamivir–RNA = 1.38 × 104 M−1. The drug interaction induces a partial B to A-DNA transition, while RNA remains in A-conformation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Structure - Volume 830, Issues 1–3, 30 March 2007, Pages 182–187
نویسندگان
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