کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1425329 986763 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Gene delivery with bisphosphonate-stabilized calcium phosphate nanoparticles
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
Gene delivery with bisphosphonate-stabilized calcium phosphate nanoparticles
چکیده انگلیسی

Nucleic acid drugs are promising new therapeutics, due to their possible applications in a wide variety of diseases and their strong targeting potential and associated lower off-target effects compared to conventional pharmaceuticals. However, their poor intracellular bioavailability and rapid degradation hinder their development as drugs. Therefore, efficient delivery is a major challenge. Various systems have been developed to overcome this problem. The entrapment of genetic material into nanoparticles constitutes a promising approach to increase the in vitro and in vivo transfection activity. Calcium phosphate-DNA co-precipitates have been used for gene delivery for more than 35 years and have the advantage of being nontoxic, easy to produce, and having the ability to complex nucleic acids leading to efficient transfection. Conventional synthetic methods yield particles that are only stable for a short period of time. Herein is proposed a versatile, surfactant-free method to stabilize calcium phosphate-DNA nanoparticles based on the use of poly(ethylene glycol)-functionalized bisphosphonate. The strength of the interaction between the bisphosphonate and the calcium phosphate enabled the formation of stable, but bioresorbable particles of around 200 nm, which exhibited physical stability over several days. Additionally, the nanoparticles revealed good and sustained ability to transfect cells while displaying low toxicity.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Controlled Release - Volume 150, Issue 1, 28 February 2011, Pages 87–93
نویسندگان
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