کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1588853 | 1515149 | 2014 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Plasticity of human dental pulp stromal cells with bioengineering platforms: A versatile tool for regenerative medicine
ترجمه فارسی عنوان
پلاستیسیته سلول های استروما پالپ دندان انسان با سیستم های زیستی مهندسی: یک ابزار همه کاره برای پزشکی احیا کننده
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کلمات کلیدی
PDTα-MEMNGFDPSCsMSCHUVECSBM-MSCsNTFsPPPbone marrow-derived mesenchymal stromal cellsLFUSBNNTsPVASHEDsDental pulp stromal cellsFBScpsBDNF - BDNF یا فاکتور نورونزایی مشتقشده از مغز Ultrasounds - اولتراسوندTem - این استDifferentiation - تفکیکPopulation doubling time - زمان دو برابر شدن جمعیتfetal bovine serum - سرم جنین گاوHuman umbilical vein endothelial cells - سلول های اندوتلیالی ورید ناف انسانStem cells - سلول های بنیادیMesenchymal stromal cells - سلولهای استرومایی مزانشیمی یا سلول های بنیادی مزانشیمیnerve growth factor - فاکتور رشد عصبBrain-derived neurotrophic factor - فاکتور نوروتروفی مشتق شده از مغزNTFs, Neurotrophic factors - فاکتورهای رشد عصبیSEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیBioengineering - مهندسی زیستیScanning electron microscopy - میکروسکوپ الکترونی روبشیTransmission electron microscopy - میکروسکوپ الکترونی عبوریBoron nitride nanotubes - نانولوله های نیترید بورDental pulp - پالپ دندان یا مغز دندانRegenerative medicine - پزشکی ترمیمیplatelet poor plasma - پلاکت پلاسمای ضعیفPoly(vinyl alcohol) - پلی وینیل الکل)Gelatin - ژلاتین
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
دانش مواد (عمومی)
چکیده انگلیسی
In recent years, human dental pulp stromal cells (DPSCs) have received growing attention due to their characteristics in common with other mesenchymal stem cells, in addition to the ease with which they can be harvested. In this study, we demonstrated that the isolation of DPSCs from third molar teeth of healthy individuals allowed the recovery of dental mesenchymal stem cells that showed self-renewal and multipotent differentiation capability. DPSCs resulted positive for CD73, CD90, CD105, STRO-1, negative for CD34, CD45, CD14 and were able to differentiate into osteogenic and chondrogenic cells. We also assayed the angiogenic potential of DPSCs, their capillary tube-like formation was assessed using an in vitro angiogenesis assay and the uptake of acetylated low-density lipoprotein was measured as a marker of endothelial function. Based on these results, DPSCs were capable of differentiating into cells with phenotypic and functional features of endothelial cells. Furthermore, this study investigated the growth and differentiation of human DPSCs under a variety of bioengineering platforms, such as low frequency ultrasounds, tissue engineering and nanomaterials. DPSCs showed an enhanced chondrogenic differentiation under ultrasound application. Moreover, DPSCs were tested on different scaffolds, poly(vinyl alcohol)/gelatin (PVA/G) sponges and human plasma clots. We showed that both PVA/G and human plasma clot are suitable scaffolds for adhesion, growth and differentiation of DPSCs toward osteoblastic lineages. Finally, we evaluated the interactions of DPSCs with a novel class of nanomaterials, namely boron nitride nanotubes (BNNTs). From our investigation, DPSCs have appeared as a highly versatile cellular tool to be employed in regenerative medicine.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Micron - Volume 67, December 2014, Pages 155-168
Journal: Micron - Volume 67, December 2014, Pages 155-168
نویسندگان
Serena Barachini, Serena Danti, Simone Pacini, Delfo D'Alessandro, Vittoria Carnicelli, Luisa Trombi, Stefania Moscato, Claudio Mannari, Silvia Cei, Mario Petrini,