کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1669970 | 1008893 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecularly imprinted polymer for the recognition of biological warfare agent staphylococcal enterotoxin B based on Surface Plasmon Resonance
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
فناوری نانو (نانو تکنولوژی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Molecularly imprinted polymers (MIPs) embody a new class of materials possessing high selectivity and affinity for the target molecules. The aim of the present work is to explore the feasibility of employing the MIP as sensing material for the detection of Staphylococcal enterotoxin B (SEB) by Surface Plasmon Resonance (SPR). In this context, the MIP film was prepared by in-situ electropolymerization of 3-aminophenylboronicacid (3-APBA) on the bare gold chip in the presence of SEB molecule. The MIP of SEB exhibited a linear response from 3.2Â fM to 25.6Â fM (r2Â =Â 0.99) with a detection limit of 0.05Â fM. KD (equilibrium constant) and Bmax (maximum binding capacity of analyte) were calculated by using kinetic evaluation software and found to be 24Â fM and 71, respectively and change in Gibb's free energy (âG) value calculated using Vant's Hoff equation was found to be â77.54Â kJ/mol. Interference study was performed with the homologs of SEB such as SEA and SEC in order to know the selectivity efficiency of SEB MIP and were found to be 23.57% and 23.43%, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Thin Solid Films - Volume 519, Issue 3, 30 November 2010, Pages 1115-1121
Journal: Thin Solid Films - Volume 519, Issue 3, 30 November 2010, Pages 1115-1121
نویسندگان
Garima Gupta, Pawan K. Singh, M. Boopathi, D.V. Kamboj, Beer Singh, R. Vijayaraghavan,