کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1676527 | 1518101 | 2006 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: An efficient matrix that resists the nonspecific adsorption of protein to fabricate carbohydrate arrays on silicon An efficient matrix that resists the nonspecific adsorption of protein to fabricate carbohydrate arrays on silicon](/preview/png/1676527.png)
Efficient matrix that resists the nonspecific adsorption of proteins from the contacting phosphate buffer silane (PBS) solution has been explored to fabricate carbohydrate arrays on silicon wafer. p-Vinylbenzyllactonoamide (VLA) was immobilized onto silicon through Si–C bond. The VLA-covered substrate was then photolithographically micropatterned through a photomask by ultraviolet (UV) irradiation. The VLA-covered regions recognized specifically the lactose-binding lectin in PBS solution. On the other hand, the UV-irradiated regions, i.e., SiOx surface, prevented the nonspecific adsorption of the lectin from the contacting PBS solution. Several control experiments have demonstrated that the resistant-ability of SiOx surface as the matrix was comparable to the best of known system for resisting the nonspecific adsorption.
Journal: Thin Solid Films - Volume 499, Issues 1–2, 21 March 2006, Pages 213–218