کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1761541 | 1019652 | 2010 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Targeted Gene Transfection from Microbubbles into Vascular Smooth Muscle Cells Using Focused, Ultrasound-Mediated Delivery
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
فیزیک و نجوم
آکوستیک و فرا صوت
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Targeted Gene Transfection from Microbubbles into Vascular Smooth Muscle Cells Using Focused, Ultrasound-Mediated Delivery Targeted Gene Transfection from Microbubbles into Vascular Smooth Muscle Cells Using Focused, Ultrasound-Mediated Delivery](/preview/png/1761541.png)
چکیده انگلیسی
We investigated a method for gene delivery to vascular smooth muscle cells using ultrasound triggered delivery of plasmid DNA from electrostatically coupled cationic microbubbles. Microbubbles carrying reporter plasmid DNA were acoustically ruptured in the vicinity of smooth muscle cells in vitro under a range of acoustic pressures (0 to 950 kPa) and pulse durations (0 to 100 cycles). No effect on gene transfection or viability was observed from application of microbubbles, DNA or ultrasound alone. Microbubbles in combination with ultrasound (500-kPa, 1-MHz, 50-cycle bursts at a pulse repetition frequency [PRF] of 100 Hz) significantly reduced viability both with DNA (53 ± 27%) and without (19 ± 8%). Maximal gene transfection (â¼1% of cells) occurred using 50-cycle, 1-MHz pulses at 300 kPa, which resulted in 40% viability of cells. We demonstrated that we can locally deliver DNA to vascular smooth muscle cells in vitro using microbubble carriers and focused ultrasound. (E-mail: jh7fj@virginia.edu)
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ultrasound in Medicine & Biology - Volume 36, Issue 9, September 2010, Pages 1470-1480
Journal: Ultrasound in Medicine & Biology - Volume 36, Issue 9, September 2010, Pages 1470-1480
نویسندگان
Linsey C. Phillips, Alexander L. Klibanov, Brian R. Wamhoff, John A. Hossack,