کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1911335 | 1046812 | 2006 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Antiproliferative effects of tocopherols (vitamin E) on murine glioma C6 cells: homologue-specific control of PKC/ERK and cyclin signaling
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کلمات کلیدی
RXRPBSFITCRetinoid X receptorPKCPXRCOX-2PPARγTocopherols - توکوفرولCancer - سرطانC6 cells - سلولهای C6Cyclooxygenase-2 - سیکلوکوکسیژناز2Cyclins - سیکلین هاCell signaling - سیگنالینگ سلولیfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریVitamin E - ویتامین E Protein kinase C - پروتئین کیناز سیPropidium iodide - پروتئین یدیدCell cycle - چرخه سلولیperoxisome proliferator activated receptor γ - گیرنده پروتئینزا پراکسیوم فعال γPregnane X receptor - گیرنده پیش گران X
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
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چکیده انگلیسی
Chemoprevention strategies for brain tumors (specifically gliomas) are few and surprisingly poorly investigated. We have studied the effects of tocopherols (TOCs; vitamin E) on proliferation and death processes of murine glioma C6 cells. These vitamers showed different cell uptake and concentration- and time-dependent inhibitory effects on cell growth that were significant at the lowest concentrations tested (1-10 μM). However, the inhibitory potency of TOCs seemed to reflect at least in part their actual cell concentrations at steady state, with the order of magnitude γ-TOC â¥Â α-TOC > δ-TOC â
 β-TOC. Moreover, for extracellular concentrations â¥10 μM, TOCs also showed a significant cytotoxic effects due mainly to necrosis, while apoptosis was negligible. γ-TOC (the form showing preferential cell uptake and lowest unspecific cytotoxicity) was the most effective inhibitor of cell cycle progression (arrest in G0/G1 phase) leading to lowered expression of cyclin E and cyclin-dependent kinases 2 and 4 and overexpression of p27 (specific inhibitor of S-phase entering). According to these signals, activated ERK1/2 and PKC upstream and Rb phosphorylation downstream were decreased. In conclusion, within TOCs the gamma form exerts the most potent and specific control of cell cycle progression in C6 cells (cytostatic effect). This suggests a chemopreventive role of this form of vitamin E in gliomas.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 41, Issue 3, 1 August 2006, Pages 464-472
Journal: Free Radical Biology and Medicine - Volume 41, Issue 3, 1 August 2006, Pages 464-472
نویسندگان
Michele Betti, Andrea Minelli, Barbara Canonico, Pasqualina Castaldo, Simona Magi, M. Cristina Aisa, Marta Piroddi, Valentina Di Tomaso, Francesco Galli,