کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1912016 | 1046856 | 2006 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Free zinc inhibits transport of vitamin C in differentiated HL-60 cells during respiratory burst
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کلمات کلیدی
3,3′-dipropylthiadicarbocyanine iodideH2DCF-DAPhorbol-12-myristate-13-acetateDcfDPIFCCP2′,7′-dichlorodihydrofluorescein diacetate - 2 '، 7'-dichlorodihydrofluorescein diacetate2′,7′-dichlorofluorescein - 2 '، 7'-dichlorofluoresceinMembrane depolarization - depolarization غشاءDiSC3(5) - DiSC3 (5)PMA - LDC هاAscorbate - آسکوربات یا ویتامین ثDehydroascorbic acid - اسید DehydroascorbicNADPH oxidase - اکسیداز NADPH DHA - دوکوساهگزائنوئیک اسیدdiphenylene iodonium chloride - دی فینیلن یون کلریدFree radical - رادیکالهای آزاد SOD - سدSuperoxide - سوپر اکسیدSuperoxide dismutase - سوکسوکس دیسموتازcarbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone - کربونیل سیانید 4- (trifluoromethoxy) phenylhydrazone
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Free zinc inhibits transport of vitamin C in differentiated HL-60 cells during respiratory burst Free zinc inhibits transport of vitamin C in differentiated HL-60 cells during respiratory burst](/preview/png/1912016.png)
چکیده انگلیسی
Zinc is an essential trace element for the immune system. It is known to be essential for highly proliferating cells, especially for cells of the immune system. However, zinc and other divalent cations are known to inhibit the human neutrophilic NADPH oxidase. Differentiated HL-60 cells were found to accumulate large quantities of vitamin C (ascorbate) after activation of the NADPH oxidase by phorbol esters (PMA). This increase in vitamin C transport is due to the generation of superoxide and subsequent oxidation of ascorbate to dehydroascorbate (DHA) which is preferentially taken up by the cells. We found that zinc reversibly inhibits both PMA-stimulated ascorbate uptake and superoxide generation with a half-maximal effect at 20 μM of free zinc ions. Higher residual extracellular ascorbate concentrations were measured with increasing zinc concentrations, indicating that less ascorbate was oxidized and taken up by the cells. When the fluorescent dye diSC3(5) was used to monitor shifts in membrane potential, we found that depolarization with PMA was prolonged after preincubation of the cells with zinc. Suppression of the respiratory burst as well as inhibition of the uptake of the antioxidant vitamin C may disturb the balance between oxidative damage of invading particles and antioxidant protection in activated neutrophils.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 40, Issue 3, 1 February 2006, Pages 436-443
Journal: Free Radical Biology and Medicine - Volume 40, Issue 3, 1 February 2006, Pages 436-443
نویسندگان
Hildegard Laggner, Katharina Phillipp, Hans Goldenberg,