کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1923114 | 1535847 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cholesterol: A modulator of the phagocyte NADPH oxidase activity - A cell-free study
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کلمات کلیدی
PMSFPtdIns(3)Pmembrane fractionsPhox homology domainPtdIns(3,4)P2Cytochrome b558dithiotreitolfMLPCytCMβCDNADPHDTTIPTGPBSGTPHEPESflavin adenine dinucleotideguanosine-5′-triphosphate - guanosine-5'-triphosphateROS - ROSEDTA - اتیلن دی آمین تترا استیک اسید Ethylenediaminetetraacetic acid - اتیلینیدامین تتراستیک اسیدArachidonic acid - اسید آراشیدونیکSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدSodium dodecyl sulfate polyacrylamide gel electrophoresis - الکتروفورز ژل پلی اتیل آمید سدیم دودسیل سولفاتNADPH oxidase - اکسیداز NADPH FAD - بدSuperoxide production - تولید سوپر اکسیدcytochrome c - سیتوکروم سیCell-free system - سیستم بدون سلولFormyl-methionyl-leucyl-phenylalanine - فرمول متیونیل-لوسیل-فنیل آلانینphosphate buffer saline - فسفات بافر شورPhenylmethanesulfonyl fluoride - فنیل متیل سولفونیل فلورایدLipid raft - قایق های چربlow density lipoprotein - لیپوپروتئین چگالی کمLDL - لیپوپروتئین کم چگالی(کلسترول بد)methyl-β-cyclodextrin - متیل-β-سیکلوکودکسترینreduced β-nicotinamide adenine dinucleotide phosphate - کاهش α-نیکوتین آمید آدنین دینکلوتید فسفاتcholesterol - کلسترولReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Cholesterol: A modulator of the phagocyte NADPH oxidase activity - A cell-free study Cholesterol: A modulator of the phagocyte NADPH oxidase activity - A cell-free study](/preview/png/1923114.png)
چکیده انگلیسی
The NADPH oxidase Nox2, a multi-subunit enzyme complex comprising membrane and cytosolic proteins, catalyzes a very intense production of superoxide ions O2
- â, which are transformed into other reactive oxygen species (ROS). In vitro, it has to be activated by addition of amphiphiles like arachidonic acid (AA). It has been shown that the membrane part of phagocyte NADPH oxidase is present in lipid rafts rich in cholesterol. Cholesterol plays a significant role in the development of cardio-vascular diseases that are always accompanied by oxidative stress. Our aim was to investigate the influence of cholesterol on the activation process of NADPH oxidase. Our results clearly show that, in a cell-free system, cholesterol is not an efficient activator of NADPH oxidase like arachidonic acid (AA), however it triggers a basal low superoxide production at concentrations similar to what found in neutrophile. A higher concentration, if present during the assembly process of the enzyme, has an inhibitory role on the production of O2
- â. Added cholesterol acts on both cytosolic and membrane components, leading to imperfect assembly and decreasing the affinity of cytosolic subunits to the membrane ones. Added to the cytosolic proteins, it retains their conformations but still allows some conformational change induced by AA addition, indispensable to activation of NADPH oxidase.
- â, which are transformed into other reactive oxygen species (ROS). In vitro, it has to be activated by addition of amphiphiles like arachidonic acid (AA). It has been shown that the membrane part of phagocyte NADPH oxidase is present in lipid rafts rich in cholesterol. Cholesterol plays a significant role in the development of cardio-vascular diseases that are always accompanied by oxidative stress. Our aim was to investigate the influence of cholesterol on the activation process of NADPH oxidase. Our results clearly show that, in a cell-free system, cholesterol is not an efficient activator of NADPH oxidase like arachidonic acid (AA), however it triggers a basal low superoxide production at concentrations similar to what found in neutrophile. A higher concentration, if present during the assembly process of the enzyme, has an inhibitory role on the production of O2
- â. Added cholesterol acts on both cytosolic and membrane components, leading to imperfect assembly and decreasing the affinity of cytosolic subunits to the membrane ones. Added to the cytosolic proteins, it retains their conformations but still allows some conformational change induced by AA addition, indispensable to activation of NADPH oxidase.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Redox Biology - Volume 3, 2014, Pages 16-24
Journal: Redox Biology - Volume 3, 2014, Pages 16-24
نویسندگان
Rawand Masoud, Tania Bizouarn, Chantal Houée-Levin,