کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1928133 1050313 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel cytoplasmic tail motif regulates mouse corin expression on the cell surface
ترجمه فارسی عنوان
یک موتیف دم جدید سیتوپلاسمی تنظیم بیان موش سوری بر روی سطح سلول را تنظیم می کند
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


• Corin is a transmembrane protease that activates the natriuretic peptides.
• Cell surface expression is important for corin zymogen activation.
• A novel cytoplasmic motif regulates corin cell surface expression and activation.

Type II transmembrane serine proteases (TTSPs) are important in many biological processes. Cell surface expression is critical for TTSP activation and function. To date, the mechanism underlying TTSP cell surface expression is poorly understood. Corin is a TTSP and acts as the pro-atrial natriuretic peptide convertase that is essential for sodium homeostasis and normal blood pressure. In this study, we investigated how cytoplasmic tail sequences may regulate corin expression and activation on the cell surface. By site-directed mutagenesis, we made mouse corin proteins with truncations or point-mutations in the cytoplasmic tail. We expressed the mutants in transfected HEK293 cells and analyzed corin cell surface expression and activation by Western blotting and flow cytometry. We found that corin truncation mutants lacking a Lys-Phe-Gln sequence at residues 71–73 had higher levels of cell surface expression and activation compared with that in wild-type corin. When Lys-71, Phe-72 and Gln-73 residues were mutated together, but not individually, in corin with the full-length cytoplasmic tail, increased levels of cell surface expression and zymogen activation were also observed. These results indicate that residues Lys-71, Phe-72 and Gln-73 serve as a novel retention motif in the intracellular pathway to regulate corin cell surface expression and activation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 465, Issue 1, 11 September 2015, Pages 152–158
نویسندگان
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