کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1928297 1050343 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Human DNA methyltransferase gene-transformed yeasts display an inducible flocculation inhibited by 5-aza-2′-deoxycytidine
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Human DNA methyltransferase gene-transformed yeasts display an inducible flocculation inhibited by 5-aza-2′-deoxycytidine
چکیده انگلیسی


• Transformation of human DNMT genes causes flocculation in the budding yeast.
• The flocculations in the DNMT genes transformants are inhibited by DNMT inhibitors.
• The flocculations in the DNMT genes transformants might be mediated by FLO1 gene.
• This is the first study to engineered yeasts respond to DNMT inhibitors.

Mammalian DNA methyltransferases (DNMTs) play an important role in establishing and maintaining the proper regulation of epigenetic information. However, it remains unclear whether mammalian DNMTs can be functionally expressed in yeasts, which probably lack endogenous DNMTs. We cotransformed the budding yeast Saccharomyces cerevisiae with the human DNMT1 gene, which encodes a methylation maintenance enzyme, and the DNMT3A/3B genes, which encode de novo methylation enzymes, in an expression vector also containing the GAL1 promoter, which is induced by galactose, and examined the effects of the DNMT inhibitor 5-aza-2′-deoxycytidine (5AZ) on cell growth. Transformed yeast strains grown in galactose- and glucose-containing media showed growth inhibition, and their growth rate was unaffected by 5AZ. Conversely, 5AZ, but not 2′-deoxycytidine, dose-dependently interfered with the flocculation exhibited by DNMT-gene transformants grown in glucose-containing medium. Further investigation of the properties of this flocculation indicated that it may be dependent on the expression of a Flocculin-encoding gene, FLO1. Taken together, these findings suggest that DNMT-gene transformed yeast strains functionally express these enzymes and represent a useful tool for in vivo screening for DNMT inhibitors.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 456, Issue 2, 9 January 2015, Pages 689–694
نویسندگان
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