کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1931232 | 1050546 | 2010 | 5 صفحه PDF | دانلود رایگان |

The hypothesis, that structural deterioration of cytochrome c oxidase (CcO) is a causal factor in the age-related decline in mitochondrial respiratory activity and an increase in H2O2 generation, was tested in Drosophila melanogaster. CcO activity and the levels of seven different nuclear DNA-encoded CcO subunits were determined at three different stages of adult life, namely, young-, middle-, and old-age. CcO activity declined progressively with age by 33%. Western blot analysis, using antibodies specific to Drosophila CcO subunits IV, Va, Vb, VIb, VIc, VIIc, and VIII, indicated that the abundance these polypeptides decreased, ranging from 11% to 40%, during aging. These and previous results suggest that CcO is a specific intra-mitochondrial site of age-related deterioration, which may have a broad impact on mitochondrial physiology.
Research highlights
► Cytochrome c oxidase loses catalytic activity during the aging process.
► Abundance of seven nuclear-encoded subunits of cytochrome c oxidase decreased with age in Drosophila.
► Cytochrome c oxidase is specific intra-mitochondrial site of age-related deterioration.
Journal: Biochemical and Biophysical Research Communications - Volume 401, Issue 1, 8 October 2010, Pages 64–68