کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1934864 1050652 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Interaction of poly(rC)-binding protein 2 domains KH1 and KH3 with coxsackievirus RNA
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Interaction of poly(rC)-binding protein 2 domains KH1 and KH3 with coxsackievirus RNA
چکیده انگلیسی

Recombinant hnRNP K-homology (KH) domains 1 and 3 of the poly(rC)-binding protein (PCBP) 2 were purified and assayed for interaction with coxsackievirus B3 RNA in electrophoretic mobility shift assays using in vitro transcribed RNAs which represent signal structures of the 5′-nontranslated region. KH domains 1 and 3 interact with the extended cloverleaf RNA and domain IV RNA of the internal ribosome entry site (IRES). KH1 but not KH3 interacts with subdomain IV/C RNA, whereas KH3 interacts with subdomain IV/B. All in vitro results are consistent with yeast three-hybrid experiments performed in parallel. The data demonstrate interaction of isolated PCBP2 KH1 and KH3 domains to four distinct target sites within the 5′-nontranslated region of the CVB3 genomic RNA.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 377, Issue 2, 12 December 2008, Pages 500–503
نویسندگان
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