کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1937508 1050717 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The role of endothelial glycocalyx components in mechanotransduction of fluid shear stress
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
The role of endothelial glycocalyx components in mechanotransduction of fluid shear stress
چکیده انگلیسی

The surface of endothelial cells is decorated with a wide variety of membrane-bound macromolecules that constitute the glycocalyx. These include glycoproteins bearing acidic oligosaccharides with terminal sialic acids (SA), and proteoglycans with their associated glycosaminoglycan that include: heparan sulfate (HS), chondroitin sulfate (CS), and hyaluronic acid (HA). In this study, enzymes were used to selectively degrade glycocalyx components from the surface of bovine aortic endothelial cells and the effects of these alterations on fluid shear-induced nitric oxide (NO) and prostacyclin (PGI2) production were determined. Depletion of HS, HA, and SA, but not CS, blocked shear-induced NO production. Surprisingly, the same enzyme depletions that blocked NO production had no influence on shear-induced PGI2 production. The results may be interpreted in terms of a glypican–caveolae–eNOS mechanism for shear-induced NO transduction, with PGI2 being transduced in basal adhesion plaques that sense the same reaction stress whether the glycocalyx is intact or not.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 355, Issue 1, 30 March 2007, Pages 228–233
نویسندگان
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