کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1946385 | 1054224 | 2014 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression of zebrafish anterior gradient 2 in the semicircular canals and supporting cells of otic vesicle sensory patches is regulated by Sox10
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
EGFRPDIHmgDICSOX10RT-PCRFGFERKAGR2hpfCLSelectrophoretic mobility shift assays - آزمایشات تحرک تحرک الکتروفورزColorless - بی رنگhour post-fertilization - ساعت پس از لقاحEMSA یا electrophoretic mobility shift assay - سنجش تغییر تحرک الکتروفورتیکendoplasmic reticulum - شبکه آندوپلاسمی fibroblast growth factor - فاکتور رشد فیبروبلاستpolymerase chain reaction - واکنش زنجیره ای پلیمرازreverse transcription polymerase chain reaction - واکنش زنجیره ای پلیمراز رونویسی معکوسPCR - واکنش زنجیرهٔ پلیمرازOtic vesicle - ویسکوزیتهprotein disulfide isomerase - پروتئین دیسولفید ایزومرازdifferential interference contrast - کنتراست تداخل دیفرانسیلextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولیAnterior gradient 2 - گرادیان قدام 2high mobility group - گروه تحرک بالاZebrafish - گورخرماهیEpidermal growth factor receptor - گیرنده فاکتور رشد اپیدرمال
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
AGR2 is a member of the protein disulfide isomerase (PDI) family, which is implicated in cancer cell growth and metastasis, asthma, and inflammatory bowel disease. Despite the contributions of this protein to several biological processes, the regulatory mechanisms controlling expression of the AGR2 gene in different organs remain unclear. Zebrafish anterior gradient 2 (agr2) is expressed in several organs, including the otic vesicles that contain mucus-secreting cells. To elucidate the regulatory mechanisms controlling agr2 expression in otic vesicles, we generated a Tg(â 6.0 k agr2:EGFP) transgenic fish line that expressed EGFP in a pattern recapitulating that of agr2. Double immunofluorescence studies were used to demonstrate that Agr2 and GFP colocalize in the semicircular canals and supporting cells of all sensory patches in the otic vesicles of Tg(â 6.0 k agr2:EGFP) embryos. Transient/stable transgenic analyses coupled with 5â²-end deletion revealed that a 100 bp sequence within the â 2.6 to â 2.5 kbp region upstream of agr2 directs EGFP expression specifically in the otic vesicles. Two HMG-binding motifs were detected in this region. Mutation of these motifs prevented EGFP expression. Furthermore, EGFP expression in the otic vesicles was prevented by knockdown of the sox10 gene. This corresponded with decreased agr2 expression in the otic vesicles of sox10 morphants during different developmental stages. Electrophoretic mobility shift assays were used to show that Sox10 binds to HMG-binding motifs located within the â 2.6 to â 2.5 kbp region upstream of agr2. These results demonstrate that agr2 expression in the otic vesicles of zebrafish embryos is regulated by Sox10.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms - Volume 1839, Issue 6, June 2014, Pages 425-437
Journal: Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms - Volume 1839, Issue 6, June 2014, Pages 425-437
نویسندگان
Chih-Hao Tang, Yun-Ren Lai, Yi-Chung Chen, Chen-Hsiu Li, Yu-Fen Lu, Hung-Yen Chen, Huang-Wei Lien, Chung-Hsiang Yang, Chang-Jen Huang, Chen-Yi Wang, Cheng-Fu Kao, Sheng-Ping L. Hwang,