کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1950156 | 1537804 | 2007 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Human glycolipid transfer protein-Intracellular localization and effects on the sphingolipid synthesis
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PoPCphenylmethanesulphonylfluorideGLTPLacCerGSLSGalCerGlcCerPBSPMSFTLCGlycolipid transfer proteinDTT1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine - 1-پالمیتویل-2-اولئویل-اسن-گلیسرو-3-فسفوشولینRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهsphingomyelin - اسفنگومیلینresonance energy transfer - انتقال انرژی رزونانسSubcellular fractionation - تقسیم بندی زیر سلولیRET - حقendoplasmic reticulum - شبکه آندوپلاسمی Phosphate buffered saline - فسفات بافر شورlactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH lactosylceramide - لاکتوزیلسرامیدConfocal microscopy - میکروسکوپ کانفوکالthin-layer chromatography - کروماتوگرافی نازک لایهgalactosylceramide - گالاکتوسیلسرامیدglucosylceramide - گلوکوزیلسرامیدGlycosphingolipids - گلیسفینگولایپید ها
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Human glycolipid transfer protein-Intracellular localization and effects on the sphingolipid synthesis Human glycolipid transfer protein-Intracellular localization and effects on the sphingolipid synthesis](/preview/png/1950156.png)
چکیده انگلیسی
Glycolipid transfer proteins (GLTPs) are small proteins that specifically transfer glycolipids from one bilayer membrane to another in vitro. However, the precise biological function is still unknown. In this study the intracellular distribution of GLTP was determined. We have used several independent methods, including differential and discontinuous density gradient centrifugation, plasma membrane permeabilization and confocal microscopy imaging, and we demonstrate that GLTP has a cytosolic location. The GLTP is not located in the Golgi apparatus, endoplasmic reticulum, nucleus, lysosomes, mitochondria or peroxisomes in HeLa cells. We have also used a fluorescence resonance energy transfer assay to detect transfer of fluorescently labeled BODIPY-glucosylceramide in the cytosolic fraction from both wild-type and GLTP-overexpressing HeLa cells. Furthermore, we have studied de novo sphingolipid changes in cells overexpressing GLTP using sphinganine metabolic labeling. The results show a significant increase in the synthesis of glucosylceramide (GlcCer) and a decrease in the sphingomyelin (SM) synthesis. However, no changes were detected in the de novo sphingolipid synthesis in GLTP-knockdown cells compared to control cells. We propose that GLTP is not likely involved in the de novo synthesis of glycosphingolipids, but could rather have a role as a glycolipid sensor for the cellular levels of glucosylceramide.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1771, Issue 11, November 2007, Pages 1353-1363
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1771, Issue 11, November 2007, Pages 1353-1363
نویسندگان
Jessica Tuuf, Peter Mattjus,