کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1950619 | 1055673 | 2014 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Regulation of cell proliferation by nucleocytoplasmic dynamics of postnatal and embryonic exon-II-containing MBP isoforms
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کلمات کلیدی
PFAGolli5-bromo-2′-deoxy-uridineN/CRFPLMBCrm1PLLPDGFPABFCSFGFFRAPGFPMBPCDKPBSnESmAbBSA - BSAC/N - C / Nbovine serum albumin - آلبومین سرم گاوMonoclonal antibody - آنتی بادی مونوکلونالPolyclonal antibody - آنتی بادی های پلی کلونالOligodendrocyte - الیگودندروسیتSerum-free - بدون سرمBrdU - بروموداکسی اوریدینHigh density - تراکم بالاLow density - تراکم کمProliferation - ترویجNucleocytoplasmic shuttling - تعویض نوکلئوسیت پالسمائیCNS - دستگاه عصبی مرکزیRoom temperature - دمای اتاقfetal calf serum - سرم گوساله جنینOligodendrocyte progenitor cell - سلول پیش گیاه Oligodendrocytecentral nervous system - سیستم عصبی مرکزیnuclear export signal - سیگنال صادرات هسته ایplatelet-derived growth factor - فاکتور رشد حاصل از پلاکتfibroblast growth factor - فاکتور رشد فیبروبلاستfluorescence recovery after photobleaching - فلوئورسانس پس از فوتوبلاسیکLeptomycin B - لپتومایسین BPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریchromosome region maintenance 1 - نگهداری منطقه کروموزوم 1paraformaldehyde - پارافرمالدهیدgreen fluorescent protein - پروتئین فلورسنت سبزred fluorescent protein - پروتئین فلورسنت قرمزMyelin basic protein - پروتئین پایه میلینPoly-l-lysine - پلی-لیزینcyclin-dependent kinase - کییناز وابسته به سیکلین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The only known structural protein required for formation of myelin, produced by oligodendrocytes in the central nervous system, is myelin basic protein (MBP). This peripheral membrane protein has different developmentally-regulated isoforms, generated by alternative splicing. The isoforms are targeted to distinct subcellular locations, which is governed by the presence or absence of exon-II, although their functional expression is often less clear. Here, we investigated the role of exon-II-containing MBP isoforms and their link with cell proliferation. Live-cell imaging and FRAP analysis revealed a dynamic nucleocytoplasmic translocation of the exon-II-containing postnatal 21.5-kDa MBP isoform upon mitogenic modulation. Its nuclear export was blocked upon treatment with leptomycin B, an inhibitor of nuclear protein export. Next to the postnatal MBP isoforms, embryonic exon-II-containing MBP (e-MBP) is expressed in primary (immature) oligodendrocytes. The e-MBP isoform is exclusively present in OLN-93 cells, a rat-derived oligodendrocyte progenitor cell line, and interestingly, also in several non-CNS cell lines. As seen for postnatal MBPs, a similar nucleocytoplasmic translocation upon mitogenic modulation was observed for e-MBP. Thus, upon serum deprivation, e-MBP was excluded from the nucleus, whereas re-addition of serum re-established its nuclear localization, with a concomitant increase in proliferation. Knockdown of MBP by shRNA confirmed a role for e-MBP in OLN-93 proliferation, whereas the absence of e-MBP similarly reduced the proliferative capacity of non-CNS cell lines. Thus, exon-II-containing MBP isoforms may regulate cell proliferation via a mechanism that relies on their dynamic nuclear import and export, which is not restricted to the oligodendrocyte lineage.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1843, Issue 3, March 2014, Pages 517-530
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1843, Issue 3, March 2014, Pages 517-530
نویسندگان
Hande Ozgen, Nicoletta Kahya, Jenny C. de Jonge, Graham S.T. Smith, George Harauz, Dick Hoekstra, Wia Baron,