کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1964797 | 1058598 | 2010 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sphingosine kinase-1/S1P1 signalling axis negatively regulates mitogenic response elicited by PDGF in mouse myoblasts
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کلمات کلیدی
DMSPDGFS1PdimethylsphingosinePTXS1PRFCSPAGEPDGFR-βS1P1SDSDMEMBSA - BSADulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده Dulbeccoshort interfering RNA - RNA تداخل کوتاهsiRNA - siRNAbovine serum albumin - آلبومین سرم گاوSphingosine kinase - اسپینوزین کینازSphingosine kinase-1 - اسپینوزین کیناز-1sphingosine 1-phosphate - اسپینگزین 1-فسفاتpolyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمیدsodium dodecyl sulphate - سدیم دودسیل سولفاتfetal calf serum - سرم گوساله جنینpertussis toxin - سموم سورافنیSkeletal muscle - عضله اسکلتیC2C12 myoblasts - میوبلاست های C2C12S1P receptor - گیرنده S1P
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Sphingosine kinase-1/S1P1 signalling axis negatively regulates mitogenic response elicited by PDGF in mouse myoblasts Sphingosine kinase-1/S1P1 signalling axis negatively regulates mitogenic response elicited by PDGF in mouse myoblasts](/preview/png/1964797.png)
چکیده انگلیسی
PDGF is known to be critically implicated in skeletal muscle repair; however its molecular mechanism of action has been only marginally investigated. In this study we show that in mouse myoblasts PDGF transactivates S1P1 receptor via sphingosine kinase (SK)-1 activation and that this molecular event exerts a negative regulation of the mitogenic effect elicited by this growth factor. Indeed, pharmacological inhibition of S1P1, or its specific silencing increased PDGF-dependent cell proliferation, whereas S1P1 overexpression diminished the biological effect. Moreover, the mitogenic response to PDGF was enhanced by pharmacological inhibition of SK activity as well as specific silencing of SK1 but not SK2. Furthermore, ERK1/2 signalling pathway was found to be upstream of the observed attenuation of PDGF-induced cell proliferation. Interestingly, PDGF-directed engagement of S1P1 exerted also a positive modulatory action of the growth factor-dependent cell motility. The here highlighted dual role of S1P1-mediated signalling in response to myoblast challenge with PDGF is likely important to guarantee the fine control of the biological response to this growth factor, finalized to efficient repopulation of skeletal muscle after damage, where a tight balance between proliferation and migration of tissue progenitor cells is required.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 22, Issue 11, November 2010, Pages 1688-1699
Journal: Cellular Signalling - Volume 22, Issue 11, November 2010, Pages 1688-1699
نویسندگان
Paola Nincheri, Caterina Bernacchioni, Francesca Cencetti, Chiara Donati, Paola Bruni,