The impact of antibody selection on the detection of cardiac troponin I

► Antibody selection impacts the relative protein yield of cTn from immunoaffinity purification. ► Amino acid variation in the epitope region abolished the binding between antibody and cTnI. ► Proteolytic degradation compromised the detection of cTnI by monoclonal antibodies. ► Phosphorylation did not significantly affect the antibody recognition of cTnI.