کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1972621 | 1060279 | 2012 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sodium deoxycholate inhibits chick duodenal calcium absorption through oxidative stress and apoptosis
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کلمات کلیدی
BLMNBTNFkBGSSGNa+/Ca2 + exchangerGAPDHGPXGSHNCX1BBMCATNaDOCPMCA1bESRplasma membrane Ca2 +-ATPaseDAB3,3′-diaminobenzidine - 3،3'-diaminobenzidineBAs - BASO2− - O2-superoxide anion - آنیون سوپر اکسیدBile acids - اسیدهای صفراویBrush border membrane - برس غشای مرزnitro blue tetrazolium - تترازولیم نیترو آبیOxidative stress - تنش اکسیداتیوintestinal calcium absorption - جذب کلسیم رودهApoptosis - خزان یاختهایGastrointestinal - دستگاه گوارشElectron spin resonance - رزونانس اسپین الکترونSOD - سدSodium deoxycholate - سدیم دگزیکسولاتSuperoxide dismutase - سوکسوکس دیسموتازBasolateral membrane - غشای نازکnuclear factor kappa B - فاکتور هسته ای کاپا BCatalase - کاتالازCalbindin D28K - کالبندین D28KQuercetin - کوئرستینGlutathione - گلوتاتیونoxidized glutathione - گلوتاتیون اکسید شدهglutathione peroxidase - گلوتاتیون پراکسیدازglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
High concentrations of sodium deoxycholate (NaDOC) produce toxic effects. This study explores the effect of a single high concentration of NaDOC on the intestinal Ca2Â + absorption and the underlying mechanisms. Chicks were divided into two groups: 1) controls and 2) treated with different concentrations of NaDOC in the duodenal loop for variable times. Intestinal Ca2Â + absorption was measured as well as the gene and protein expressions of molecules involved in the Ca2Â + transcellular pathway. NaDOC inhibited the intestinal Ca2Â + absorption, which was concentration dependent. Ca2Â +-ATPase mRNA decreased by the bile salt and the same occurred with the protein expression of Ca2Â +-ATPase, calbindin D28Â k and Na+/Ca2Â + exchanger. NaDOC produced oxidative stress as judged by ROS generation, mitochondrial swelling and glutathione depletion. Furthermore, the antioxidant quercetin blocked the inhibitory effect of NaDOC on the intestinal Ca2Â + absorption. Apoptosis was also triggered by the bile salt, as indicated by the TUNEL staining and the cytochrome c release from the mitochondria. As a compensatory mechanism, enzyme activities of the antioxidant system were all increased. In conclusion, a single high concentration of NaDOC inhibits intestinal Ca2Â + absorption through downregulation of proteins involved in the transcellular pathway, as a consequence of oxidative stress and mitochondria mediated apoptosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 162, Issue 4, August 2012, Pages 397-405
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 162, Issue 4, August 2012, Pages 397-405
نویسندگان
MarÃa A. Rivoira, Ana M. Marchionatti, Viviana A. Centeno, Gabriela E. DÃaz de Barboza, MarÃa E. Peralta López, Nori G. Tolosa de Talamoni,