کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2015659 | 1541937 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Phytotoxic action of naphthoquinone juglone demonstrated on lettuce seedling roots
ترجمه فارسی عنوان
اثر فیتوتوکسید نوتووکینون جوگلون در ریشه های گیاهچه کاهو نشان داده شده است
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کلمات کلیدی
TTCMDACDPKRNSPODMAPK - MAPKMTT - MTTROS - ROSAcridine orange - آکاردین نارنجیethidium bromide - اتیدیوم برومایدTerminal deoxynucleotidyl transferase dUTP nick end labeling - ترمینال deoxynucleotidyl transferase dUTP نام نهایی پایان نامهTUNEL - تونلRoot - ریشهmalondialdehyde - مالون دی آلدهیدProgrammed cell death - مرگ برنامهریزی شده یاختهfresh weight - وزن ترPeroxidase - پراکسیدازCalcium-dependent protein kinase - پروتئین کیناز وابسته به کلسیمLettuce - کاهوreactive nitrogen species - گونه های واکنش پذیر نیتروژنReactive oxygen species - گونههای فعال اکسیژنJuglone - یوگلون
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
چکیده انگلیسی
Juglone, 5-hydroxy-1,4-naphthoquinone, is the plant secondary metabolite with allelopathic properties, which was isolated especially from the plant species belonging to family Juglandaceae A. Rich. ex Kunth (walnut family). The mechanism of phytotoxic action of juglone was investigated on lettuce seedlings Lactuca sativa L. var. capitata L. cv. Merkurion by determining its effect at different levels. We have found that juglone inhibits mitosis (mitotic index 8.5 ± 0.6% for control versus 2.2 ± 0.9% for 250 μM juglone), changes mitotic phase index with accumulation of the cells in prophase (56.5 ± 2.6% for control versus 85.3 ± 5.0% for 250 μM juglone), and decreases meristematic activity in lettuce root tips (51.07 ± 3.62% for control versus 5.27 ± 2.29% for 250 μM juglone). In addition, juglone induced creation of reactive oxygen species and changed levels of reactive nitrogen species. Amount of malondialdehyde, a product of lipid peroxidation, increased from 24.0 ± 4.0 ng gâ1 FW for control to 55.5 ± 5.4 ng gâ1 FW for 250 μM juglone. We observed also changes in cellular structure, especially changes in the morphology of endoplasmic reticulum. Reactive oxygen species induced damage of plasma membrane. All these changes resulted in the disruption of the mitochondrial membrane potential, increase in free intracellular calcium ions, and DNA fragmentation and programmed cell death that was revealed by two methods, TUNEL test and DNA electrophoresis. The portion of TUNEL-positive cells increase from 0.96 ± 0.5% for control to 7.66 ± 1.5% for 250 μM juglone. Results of the study indicate complex mechanism of phytotoxic effect of juglone in lettuce root tips and may indicate mechanism of allelopathic activity of this compound.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 84, November 2014, Pages 78-86
Journal: Plant Physiology and Biochemistry - Volume 84, November 2014, Pages 78-86
نویسندگان
Petr Babula, Veronika Vaverkova, Zuzana Poborilova, Ludmila Ballova, Michal Masarik, Ivo Provaznik,