کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2015780 | 1541938 | 2014 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparison of a compatible and an incompatible pepper-tobamovirus interaction by biochemical and non-invasive techniques: Chlorophyll a fluorescence, isothermal calorimetry and FT-Raman spectroscopy
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PMMoVCCDPS IIPSII efficiencyplant efficiency analyzerhpiPEANPQΦPSIIOECFv/Fm - Fv / FmROS - ROSLOX - اکسیژن مایعNon-photochemical quenching - خنک سازی غیر فتوشیمیاییcarotenoid cleavage dioxygenase - دیاکسیدگناز لاکتوز کاروتنوئیدhours post-inoculation - ساعت پس از تلقیحCapsicum annuum - سیب زمینیphotochemical quenching coefficient - ضریب خنک کننده فتوشیمیاییRaman spectroscopy - طیف سنجی رامانPhotosystem II - فتوسیستم 2Pepper mild mottle virus - فلفل ویروس مچاله خفیفlipoxygenase - لیپواکسیژنازHypersensitive response - پاسخ حساسیتOxygen-evolving complex - پیچیده اکسیژن تکامل یافتهReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Leaves of a pepper cultivar harboring the L3 resistance gene were inoculated with Obuda pepper virus (ObPV), which led to the appearance of hypersensitive necrotic lesions approx. 72 h post-inoculation (hpi) (incompatible interaction), or with Pepper mild mottle virus (PMMoV) that caused no visible symptoms on the inoculated leaves (compatible interaction). ObPV inoculation of leaves resulted in ion leakage already 18 hpi, up-regulation of a pepper carotenoid cleavage dioxygenase (CCD) gene from 24 hpi, heat emission and declining chlorophyll a content from 48 hpi, and partial desiccation from 72 hpi. After the appearance of necrotic lesions a strong inhibition of photochemical energy conversion was observed, which led to photochemically inactive leaf areas 96 hpi. However, leaf tissues adjacent to these inactive areas showed elevated ΦPSII and Fv/Fm values proving the advantage of chlorophyll a imaging technique. PMMoV inoculation also led to a significant rise of ion leakage and heat emission, to the up-regulation of the pepper CCD gene as well as to decreased PSII efficiency, but these responses were much weaker than in the case of ObPV inoculation. Chlorophyll b and total carotenoid contents as measured by spectrophotometric methods were not significantly influenced by any virus inoculations when these pigment contents were calculated on leaf surface basis. On the other hand, near-infrared FT-Raman spectroscopy showed an increase of carotenoid content in ObPV-inoculated leaves suggesting that the two techniques detect different sets of compounds.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 83, October 2014, Pages 267-278
Journal: Plant Physiology and Biochemistry - Volume 83, October 2014, Pages 267-278
نویسندگان
Magdalena Rys, Csilla Juhász, Ewa Surówka, Anna Janeczko, Diana Saja, István Tóbiás, Andrzej Skoczowski, Balázs Barna, Gábor Gullner,